Comparative proteomic studies in Rhodospirillum rubrum grown under different nitrogen conditions
2008 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 7, no 8, 3267-3275 p.Article in journal (Refereed) Published
Forty-four differentially expressed proteins have been identified in the photosynthetic diazotroph Rhodospirillum rubrum grown anaerobic and photoheterotrophically, with different nitrogen sources, using 2D-PAGE and MALDI-TOF, from gels containing an average of 679 ± 52 (in N+) and 619 ± 37 (in N−) protein spots for each gel. A higher level of expression was found under nitrogen-rich growth, for proteins involved in carbon metabolism (reductive tricarboxylic acid cycle, CO2 fixation, and poly-β-hydroxybutyrate metabolism) and amino acid metabolism. The key enzymes RuBisCO and α-ketoglutarate synthase were found to be present in higher amounts in nitrogen-rich conditions. Ntr and Nif regulated proteins, such as glutamine synthetase and nitrogenase, were, as expected, induced under nitrogen-fixing conditions and glutamate dehydrogenase was down regulated. A novel 2Fe-2S ferredoxin with unknown function was identified from nitrogen-fixing cultures. In addition to differential expression, two of the identified proteins revealed variable pI values in response to the nitrogen source used.
Place, publisher, year, edition, pages
2008. Vol. 7, no 8, 3267-3275 p.
Rhodospirillum rubrum, nitrogen fixation, comparative proteome, 2D-PAGE
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-14839DOI: 10.1021/pr700771uISI: 000258200400017PubMedID: 18570453OAI: oai:DiVA.org:su-14839DiVA: diva2:181359