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Structure of human argininosuccinate synthetase.
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2008 (English)In: Acta Crystallogr D Biol Crystallogr, ISSN 0907-4449, Vol. 64, no Pt 3, 279-86 p.Article in journal (Refereed) Published
Abstract [en]

Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step in both the urea and arginine-citrulline cycles. Previous studies have investigated the crystal structures of argininosuccinate synthetase from bacterial species. In this work, the first crystal structure of human argininosuccinate synthetase in complex with the substrates citrulline and aspartate is presented. The human enzyme is compared with structures of argininosuccinate synthetase from bacteria. In addition, the structure also provides new insights into the function of the numerous clinical mutations identified in patients with type I citrullinaemia (also known as classic citrullinaemia).

Place, publisher, year, edition, pages
2008. Vol. 64, no Pt 3, 279-86 p.
Keyword [en]
Adenosine Triphosphate/chemistry/metabolism, Amino Acid Sequence, Argininosuccinate Synthase/*chemistry/genetics, Binding Sites, Crystallography; X-Ray, Humans, Models; Molecular, Molecular Sequence Data, Protein Conformation, Sequence Analysis; Protein, Substrate Specificity
URN: urn:nbn:se:su:diva-14861PubMedID: 18323623OAI: diva2:181381
Available from: 2008-11-06 Created: 2008-11-06 Last updated: 2011-01-10Bibliographically approved

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Högbom, Martin
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Department of Biochemistry and Biophysics

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