Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter.
2008 (English)In: J Mol Biol, ISSN 1089-8638, Vol. 381, no 4, 860-6 p.Article in journal (Refereed) Published
The topology of bacterial inner membrane proteins is commonly determined using topology reporters such as alkaline phosphatase and green fluorescent protein fused to a series of C-terminally truncated versions of the protein in question. Here, we report a detailed topology mapping of the Escherichia coli inner membrane H(+)/Cl(-) exchange transporter ClcA. Since the 3-D structure of ClcA is known, our results provide a critical test of the reporter fusion approach and offer new insights into the ClcA folding pathway.
Place, publisher, year, edition, pages
2008. Vol. 381, no 4, 860-6 p.
Alkaline Phosphatase/metabolism, Amino Acid Sequence, Antiporters/chemistry/*metabolism, Escherichia coli/*metabolism, Escherichia coli Proteins/chemistry/*metabolism, Genes; Reporter, Green Fluorescent Proteins/metabolism, Ion Pumps/*metabolism, Membrane Proteins/*metabolism, Models; Molecular, Molecular Sequence Data, Protein Structure; Secondary, Recombinant Fusion Proteins/*metabolism
IdentifiersURN: urn:nbn:se:su:diva-14919ISI: 000258971300006PubMedID: 18590742OAI: oai:DiVA.org:su-14919DiVA: diva2:181439