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Cholesterol prevents interaction of the cell-penetrating peptide transportan with model lipid membranes
Stockholm University, Faculty of Science, Department of Neurochemistry.
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2008 (English)In: Journal of Peptide Science, ISSN 1075-2617, E-ISSN 1099-1387, Vol. 14, no 12, 1303-1308 p.Article in journal (Refereed) Published
Abstract [en]

Interaction of the cell-penetrating peptide (CPP) cysteine-transportan (Cys-TP) with model lipid membranes was examined by spin-label electron paramagnetic resonance (EPR). Membranes were labeled with lipophilic spin probes and the influence of Cys-TP on membrane structure Was studied. The influence of Cys-TP on membrane permeability was monitored by the reduction of a liposome-trapped water-soluble spin probe. Cys-TP caused lipid ordering in membranes prepared from pure dimyristoylphosphatidylcholine (DMPC) and in DMPC membranes with moderate cholesterol concentration. In addition, Cys-TP caused a large increase in permeation of DMPC membranes. In contrast, with high cholesterol content, at which model lipid membranes are in the so-called liquid-ordered phase, no effect. of Cys-TP was observed, either on Line membrane structure or on the membrane permeability. The interaction between Cys-TP and the lipid membrane therefore depends on the lipid phase. This could be of great. importance for understanding of the CPP-lipid interaction in laterally heterogeneous membranes, white it implies that the CPP-lipid interaction can be different at different points along the membrane.

Place, publisher, year, edition, pages
2008. Vol. 14, no 12, 1303-1308 p.
Keyword [en]
cell-penetrating peptide, transportan, spin-label electron paramagnetic resonance, model lipid membranes, cholesterol, lipid phase
National Category
Biological Sciences Chemical Sciences
URN: urn:nbn:se:su:diva-15049DOI: 10.1002/psc.1062ISI: 000262016400009PubMedID: 18683276OAI: diva2:181569
Available from: 2008-11-19 Created: 2008-11-19 Last updated: 2015-04-21Bibliographically approved

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Langel, Ülo
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