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Cellular traits for sodium tolerance in rice (Oryza sativa L.)
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Sylvia Lindberg)
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Sylvia Lindberg)
2008 (English)In: Plant Biotechnology, ISSN 0289577, Vol. 25, no 3, 247-255 p.Article in journal (Refereed) Published
Abstract [en]


The present review focuses on two important aspects of Na+ toxicity in rice (Oryza sativa L.), i) that Na+ stress induces different changes in cytosolic Ca2+, [Ca2+]cyt, and pH, [pH]cyt, in tolerant and sensitive cultivars, and ii) that cells from a tolerant cultivar can better maintain a low cytosolic Na+ and/or Na+/K+ ratio. Experiments with single rice protoplasts, fluorescence microscopy and specific ion-selective dyes suggest that Na+ must be sensed inside the cytosol, before any prolonged changes in [Ca2+]cyt and [pH]cyt occur. Inhibitor analyses show that Na+-induced increase in [pH]cyt in the tolerant cv. Pokkali, and a decrease in [pH]cyt in the sensitive cv. BRRI DHan29, likely are coupled to different H+-ATPases. Expression analysis of OsHKT2;1 (previous name OsHKT1), OsHKT2;2 (previous name OsHKT2) and OsVHA transcripts in rice using RT-PCR and fluorescence in situ-PCR, shows a variable and cell- specific induction in the two rice cultivars under salt stress condition. We conclude that the transient uptake of Na+, which occurs only in the tolerant cultivar, and the fast compartmentalization of Na+ into the vacuole, probably are the most important cellular traits for Na+-tolerance in rice. The low [Na+]cyt in cv. Pokkali might depend on the fast down-regulation of OsHKT2;1, causing less uptake of Na+, and fast up-regulation of the OsVHA transcript, and subsequent activation of the Na+/H+-anti-porter in the tonoplast. To decrease the cytosolic Na+/K+ ratio under Na+ toxicity, cv. Pokkali may also induce increased uptake of K+ through induction of OsHKT2;2, and other specific K+-transporter genes.

Place, publisher, year, edition, pages
2008. Vol. 25, no 3, 247-255 p.
Keyword [en]
Cytosolic calcium and pH, H+ATPase, HKT, Na-toxicity, Oryza sativa
National Category
URN: urn:nbn:se:su:diva-15293ISI: 000262639800008OAI: diva2:181813
Projekt A. In the program:Plasma membrane properties and signal transduction in plant cells under stress from: 2008-11-27 Created: 2008-11-27 Last updated: 2011-01-10Bibliographically approved

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