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SPE and HPLC/UV of resin acids in colophonium-containing products
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
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2008 (English)In: Journal of Separation Science, Vol. 31, no 15, 2784-2790 p.Article in journal (Refereed) Published
Abstract [en]

A new method, involving SPE and HPLC/UV diode-array detection (DAD), was developed for the quantification of colophonium components in different consumer products, such as cosmetics. Colophonium is a common cause of contact dermatitis since its components can oxidize into allergens on exposure to air. Three different resin acids were used as markers for native and oxidized colophonium, abietic acid (AbA), dehydroabietic acid (DeA), and 7-oxodehydroabietic acid (7-O-DeA). The SPE method, utilizing a mixed-mode hydrophobic and anion exchange retention mechanism, was shown to yield very clean extracts. The use of a urea-embedded C12 HPLC stationary phase improved the separation of the resin acids compared to common C18. Concentrations higher than 2 mg/g of both AbA and DeA were detected in wax strips. In this product also 7-O-DeA, a marker for oxidized colophonium, was detected at a level of 28 μg/g. The LODs were in the range of 7–19 μg/g and the LOQs 22–56 μg/g. The method is simple to use and can be applied on many types of technical products, not only cosmetics. For the first time, a method for technical products was developed, which separates AbA from pimaric acid

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2008. Vol. 31, no 15, 2784-2790 p.
Keyword [en]
Allergic contact dermatitis;Colophonium;HPLC/UV;Resin acids;SPE
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-17516DOI: 10.1002/jssc.200800210ISI: 000258936700004OAI: oai:DiVA.org:su-17516DiVA: diva2:184037
Available from: 2009-01-16 Created: 2009-01-16 Last updated: 2012-03-13Bibliographically approved
In thesis
1. Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
Open this publication in new window or tab >>Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The aims of the work this thesis is based upon were to develop convenient analytical procedures for determining resin acids in biological and environmental matrices, and apply them to enhance understanding of the occurrence, exposure to and uptake by exposed individuals of resin acids. Particular focus has been on the workplace environment of the Swedish wood pellets industry. Sample extraction procedures and high-performance liquid chromatography/electrospray ionisation-mass spectrometry (HPLC/ESI-MS) methodologies were developed for measuring resin acids in dust, skin and urine samples. Chromatographic separation of abietic (AA) and pimaric acid was achieved by using a polar-embedded C12 stationary phase. The HPLC/ESI-MS method avoids undesirable oxidation of AA, which was found to occur during the derivatisation step in the standard MDHS 83/2 gas chromatography/flame ionisation detection (GC/FID) methodology, leading to false observations of both AA and the oxidation product 7-oxodehydroabietic acid (7-OXO). Personal exposures to resin acids in the Swedish wood pellet production industry were found to be lower, on average, than the British Occupational Exposure Limit for rosin (50 µg/m3). The oxidised resin acid 7-OXO, was detected in both dust and skin samples indicating the presence of allergenic resin acids. A correlation between air and post-shift urinary concentrations of dehydroabietic acid (DHAA), and a trend towards an increase in urinary 7-OXO during work shifts, were also observed. Whether the increase in 7-OXO was due to direct uptake or metabolism of other resin acids cannot be concluded from the results. An efficient HPLC/UV methodology with diode-array detection was developed for screening commercial products for rosin that could be used in laboratories lacking mass spectrometers. Very high concentrations of free resin acids were detected in depilatory wax strips using the method.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2012. 60 p.
Keyword
Rosin, resin acids, HPLC/ESI-MS, air sampling, urine samples, wood pellets, wood dust
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-74448 (URN)978-91-7447-449-7 (ISBN)
Public defence
2012-04-27, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 13:00 (English)
Opponent
Supervisors
Note
At the time of doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Submitted.Available from: 2012-04-03 Created: 2012-03-12 Last updated: 2012-03-28Bibliographically approved

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