Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cadmium uptake and interaction with phytochelatins in wheat protoplasts: Cadmium uptake and interaction with phytochelatins in wheat protoplasts
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Sylvia Lindberg)
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Maria Greger)
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Maria greger)
2007 (English)In: Plant Physiology and Biochemistry: Plant Physiology and Biochemistry, Vol. 45, no 1, 47-53 p.Article in journal (Refereed) Published
Abstract [en]

In order to investigate the role of phytochelatins in short-time uptake of Cd2+ into the cytosol of wheat protoplasts, a new method was

applied, using fluorescence microscopy and the heavy metal-specific fluorescent dye, 5-nitrobenzothiazole coumarin, BTC-5N. The uptake of Cd2+ into protoplasts from 5- to 7-day-old wheat seedlings (Triticum aestivum, L. cv. Kadett) was lower in protoplasts from seedlings raised in

the presence of 1 mM CdCl2, than in the absence. Presence of CdCl2 in the cultivation medium increased the content of phytochelatins (PCs) in the protoplasts. When seedlings were raised in the presence of both Cd2+ and buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, only little PC was found in the protoplasts. Pre-treatment with BSO alone did not affect the content of PC, but inhibited that of GSH. The inhibition of GSH was independent of pre-treatment with Cd2+. Unidirectional flux analyses, using 109Cd2+, showed approximately the same uptake pattern of Cd2+ as did the fluorescence experiments showing the cytosolic uptake of Cd2+. Thus, the diminished uptake of Cd2+ into protoplasts from cadmium-pre-treated plants was not depending on PCs. Instead, it is likely that pre-treatment with Cd2+ causes a down-regulation of the short-term Cd2+ uptake, or an up-regulation of the Cd2+ extrusion. Moreover, since addition of Cd2+ to protoplasts from control plants caused a cytosol acidification, it is likely that a Cd2+/H+-antiport mechanism is involved in the extrusion of Cd2+ from these protoplasts.

Place, publisher, year, edition, pages
2007. Vol. 45, no 1, 47-53 p.
Keyword [en]
Cadmium uptake, Fluorescence microscopy, Protoplasts, Phytochelatins, Triticum aestivum
National Category
Botany
Identifiers
URN: urn:nbn:se:su:diva-19279ISI: 000245539600007OAI: oai:DiVA.org:su-19279DiVA: diva2:185803
Available from: 2007-11-01 Created: 2007-11-01 Last updated: 2011-01-11Bibliographically approved

Open Access in DiVA

No full text

Other links

http://www.botan.su.se/fysiologi
By organisation
Department of Botany
Botany

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 50 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf