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A shift in sensitivity to auxin within development of maize seedlings
St Petersburg State University. (Maria Shishova)
St Petersburg State University.
St Petersburg State Universiry. (Maria Shishova)
Stockholm University, Faculty of Science, Department of Botany. växtfysiologi. (Sylvia Lindberg)
2007 (English)In: Journal of Plant Physiology, Vol. 164, 1323-1330 p.Article in journal (Refereed) Published
Abstract [en]

The auxin-induced changes in cytosolic concentrations of Ca2+ and H+ ions were investigated in protoplasts from maize coleoptile cells at 3rd, 4th and 5th day of development of etiolated seedlings. The shifts in [Ca2+]cyt and [H+]cyt were detected by use of fluorescence microscopy in single protoplasts loaded with the tetra[acetoxymethyl]esters of the fluorescent calcium binding Fura 2, or pH-sensitive carboxy-fluorescein, BCECF, respectively. Both the auxin-induced shifts in the ion concentrations were specific to the physiologically active synthetic auxin, naphthalene-1-acetic acid (1-NAA), and not to the non-active naphthalene-2-acetic acid (2-NAA). Regardless of the age of the seedlings, the rise in [Ca2+]cyt was prior to the acidification in all investigated cases. The maximal acidification coincided with the highest amplitude of [Ca2+]cyt change, but not directly depended on the concentration of 1-NAA. Within aging of the seedlings the amplitude of auxin-induced [Ca2+]cyt elevation decreased. The shift in auxin-induced acidification was almost equal at 3rd and 4th day, but largely dropped at 5th day of development. The acidification was related to changes in the plasma

membrane H+-ATPase activity, detected as phosphate release. The decrement in amplitude of both the tested auxin-triggered reactions well coincided with the end of the physiological function of the coleoptile. Hence the primary auxin-induced increase in [Ca2+]cyt, which is supposed to be an important element of hormone signal perception

and transduction, can be used as a test for elucidation of plant cell sensitivity to auxin.

Place, publisher, year, edition, pages
2007. Vol. 164, 1323-1330 p.
Keyword [en]
Auxin, Cytoplasmic calcium, Acidification, Fluorescence microscopy, Sensitivity
National Category
URN: urn:nbn:se:su:diva-19284ISI: 000250439700008OAI: diva2:185808
Auxin signaling in plant cells
Available from: 2007-11-01 Created: 2007-11-01 Last updated: 2010-01-22Bibliographically approved

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