A NOVEL MECHANISTIC PATHWAY FOR SURFACTANT-INDUCED NOCICEPTION
2007 (English)In: 25th SSCT annual workshop, 2007Conference paper (Other (popular science, discussion, etc.))
The pain receptor transient receptor potential vanilloid type 1 (TRPV1) has been reported as one of the key components in the pain pathway. Activation of the receptor causes a Ca2+ influx with secondary effects leading to neurogenic inflammation. Here we report specific activation of TRPV1 by detergent-containing hygiene products measured as intracellular Ca2+ influxes in stably TRPV1-expressing neuroblastoma SH-SY5Y cells. Commercial hygiene products were applied to the cells in diluted solutions and stimulation was monitored during exposure as an increase in the intracellular free Ca2+ levels by using the Fura 2 fluorescence assay and semi-HTS fluorometer for 96 well plates. Co-application with the TRPV1 antagonist capsazepine revealed if the response was specific for the receptor. The response was quantified as the product induced Ca2+ influx during 2 minutes in relation to the maximum response induced by the TRPV1 agonist capsaicin. The results show that shampoos and soaps induced a TRPV1 mediated Ca2+ influx whereas children products marketed as “painless” (containing lower concentration of detergents), and conditioners (without detergents) did not induce specific TRPV1 activation. Furthermore, low concentrations of the detergent sodium lauryl sulfate (SLS) dose-dependently induced Ca2+ influx that could be addressed to TRPV1. These results reveal a novel mechanistic pathway for surfactant-induced nociception, which may be an important endpoint in in vitro test batteries, as alternatives to Draize’s rabbit eye test, for classification of mild eye irritating products.
Place, publisher, year, edition, pages
Eye irritation, TRPV1, nociception, alternative method
IdentifiersURN: urn:nbn:se:su:diva-19568OAI: oai:DiVA.org:su-19568DiVA: diva2:186092