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The single-cell transcriptional landscape of innate and adaptive lymphocytes in pediatric-onset colitis
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Number of Authors: 242023 (English)In: Cell reports medicine, ISSN 2666-3791, Vol. 4, no 5, article id 101038Article in journal (Refereed) Published
Abstract [en]

Innate lymphoid cells (ILCs) are considered innate counterparts of adaptive T cells; however, their common and unique transcriptional signatures in pediatric inflammatory bowel disease (pIBD) are largely unknown. Here, we report a dysregulated colonic ILC composition in pIBD colitis that correlates with inflammatory ac-tivity, including accumulation of naive-like CD45RA+CD62L- ILCs. Weighted gene co-expression network analysis (WGCNA) reveals modules of genes that are shared or unique across innate and adaptive lympho-cytes. Shared modules include genes associated with activation/tissue residency, naivety/quiescence, and antigen presentation. Lastly, nearest-neighbor-based analysis facilitates the identification of most in-flamedand least inflamedlymphocytes in pIBD colon with unique transcriptional signatures. Our study reveals shared and unique transcriptional signatures of colonic ILCs and T cells in pIBD. We also provide insight into the transcriptional regulation of colonic inflammation, deepening our understanding of the poten-tial mechanisms involved in pIBD.

Place, publisher, year, edition, pages
2023. Vol. 4, no 5, article id 101038
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Immunology in the medical area
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URN: urn:nbn:se:su:diva-229676DOI: 10.1016/j.xcrm.2023.101038ISI: 001005442000001PubMedID: 37160121Scopus ID: 2-s2.0-85159385818OAI: oai:DiVA.org:su-229676DiVA, id: diva2:1861141
Available from: 2024-05-27 Created: 2024-05-27 Last updated: 2024-05-27Bibliographically approved

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Czarnewski, Paulo

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Theorell, JakobCzarnewski, PauloLourda, MagdaTibbitt, Christopher AndrewNordenvall, CarolineBryceson, Yenan T.Rolandsdotter, Helena
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
Immunology in the medical area

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CiteExportLink to record
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