Spatiotemporal changes in the Holocene climate of the arid region of China were principally caused by shifts in the intensity and location of the East Asian summer monsoon (EASM). However, our knowledge of these EASM variations is limited, especially since the middle Holocene. We investigated two aeolian sedimentary sections (the Linxia/LX and Gulang/GL sections) on the western Chinese Loess Plateau (CLP), to explore the variations in the intensity and location of the EASM and their impact on the regional environment and human cultural development during the middle to late Holocene. The longitudes of these two loess sections are similar, but the GL section is located on the northern edge of the CLP, in the eastern Hexi Corridor, and it is situated further north than the LX section. The chronologies of both sections are based on accelerator mass spectrometry C-14 dating, and environmental magnetic parameters and grain size end-members were used to reconstruct their moisture histories. The results suggest that the climate at the LX section was relatively dry before 3.5 ka, after which it became wetter. In contrast, at the GL section, the climate was also relatively dry before 3.5 ka, with the wettest conditions occurring during 3.5-2 ka, but subsequently there was an abrupt shift to a dry climate. We suggest that these spatiotemporal patterns reflect the westward movement and rapid southward retreat of the EASM and the linked monsoon rainfall belt. TraCE-21 ka climate simulation results support our inference. We also found that, compared with other ancient cultures in the eastern Hexi Corrido, sites of the Shajing culture, dated to similar to 2.4-2.8 ka, are predominantly located in areas that are arid at the present-day, and relatively distant from rivers. We suggest that this distribution pattern reflected the occurrence of a humid climate during the period of the Shajing culture, thus demonstrating the impact of the EASM on cultural development in this region.