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Characterization of the antioxidative activity of novel nontoxic neuropeptides by using capillary electrophoresis.
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2006 (English)In: Electrophoresis, ISSN 0173-0835, Vol. 27, no 13, 2582-9 p.Article in journal (Refereed) Published
Abstract [en]

In the present study, we have monitored the oxidation process of novel nontoxic neuropeptides and determined its rate constants, which describe the antioxidative potential of the peptides. A capillary electrophoretic method was implemented which ensures the simultaneity of analysis of reactants and products in a short time of analysis. The rate constants of oxidation of the four novel peptides, 4-methoxy-L-tyrosinyl-gamma-L-glutamyl-L-cysteinyl-glycine (UPF1), D-serinyl-gamma-L-glutamyl-L-cysteinyl-glycine (UPF6), 4-methoxy-L-tyrosinyl-alpha-L-glutamyl-L-cysteinyl-glycine and D-serinyl-alpha-L-glutamyl-L-cysteinyl-glycine, designed by us, were compared with those of oxidation of glutathione (reduced glutathione) by using capillary electrophoresis. The second-order rate constants were similar for all peptides if the oxidation was carried out with hydrogen peroxide (k(II) = 0.208 - 0.236 x 10(3)/M.min). The rate constants were also determined for the mixtures of peptides. When the oxidation is caused by hydroxyl radical (OH*), the gamma-glutamate containing peptides (UPF1 and UPF6) exhibited two to four times higher antioxidative activity (k(II) = 4.428 and 2.152 x 10(3)/M.min, respectively). The results suggest that the antioxidative potential of the peptides studied is not determined by the formation of disulphide bridge alone.

Place, publisher, year, edition, pages
2006. Vol. 27, no 13, 2582-9 p.
Keyword [en]
Antioxidants, Electrophoresis; Capillary, Hydrogen Peroxide, Hydroxyl Radical/chemistry, Kinetics, Neuropeptides/*chemistry, Oxidation-Reduction
Identifiers
URN: urn:nbn:se:su:diva-20406DOI: doi:10.1002/elps.200500760PubMedID: 16732619OAI: oai:DiVA.org:su-20406DiVA: diva2:186932
Available from: 2007-03-21 Created: 2007-03-21 Last updated: 2011-01-11Bibliographically approved

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Jarvet, J
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Department of Biochemistry and Biophysics

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