Insulin-like growth factor type 1 upregulates uncoupling protein 3.
2001 (English)In: Biochem Biophys Res Commun, ISSN 0006-291X, Vol. 287, no 5, 1105-11 p.Article in journal (Refereed) Published
In this study the expression of uncoupling protein 3 (UCP3) and its regulation by insulin-like growth factor 1 (IGF-I) and insulin in human neuroblastoma SH-SY5Y cells were characterized. Reverse transcriptase-PCR, Western blot, and immunofluorescence analysis showed that SH-SY5Y cells express UCP3 natively. IGF-I induced a time- and concentration-dependent induction of UCP3 protein reaching a twofold expression after 72 h with 10 nM IGF-I. Extremely high insulin concentrations (860 nM) and 10 nM trIGF-I, a truncated form of IGF-I with the same affinity for the IGF-I receptor as the full-length IGF-I, but with lower activity on the insulin receptor, also upregulated UCP3. We conclude that SH-SY5Y cells express UCP3 natively and that the expression is regulated by IGF-I via the IGF-I receptor. Copyright 2001 Academic Press.
Place, publisher, year, edition, pages
2001. Vol. 287, no 5, 1105-11 p.
Carrier Proteins/biosynthesis/*genetics, Diabetes Mellitus; Type 2/metabolism, Diabetic Neuropathies/etiology, Humans, Insulin/pharmacology, Insulin-Like Growth Factor I/*pharmacology, Ion Channels, Mitochondria/metabolism, Mitochondrial Proteins, Nerve Tissue/drug effects/*metabolism, Neuroblastoma, Receptor; IGF Type 1/isolation & purification, Receptor; Insulin/isolation & purification, Tumor Cells; Cultured, Uncoupling Agents/metabolism, Up-Regulation
IdentifiersURN: urn:nbn:se:su:diva-20503PubMedID: 11587536OAI: oai:DiVA.org:su-20503DiVA: diva2:187029