Co-transduction of Sleeping Beauty Transposase and Donor Plasmid via a Cell-penetrating Peptide: A simple one-step method
2008 (English)In: International journal of peptide research and therapeutics, ISSN 1573-3904, Vol. 14, no 1, 58-63 p.Article in journal (Refereed) Published
Transposable elements have emerged as a promising candidate for human non-viral gene-therapy. The Tc1/mariner transposon Sleeping Beauty is to date one of the most efficient transposons in mammals. Sleeping Beauty transposase has so far mostly been delivered to cells via a DNA source. This might cause spontaneous integration of the transposase gene and cause fatal damage to the affected cell. Hence, it would be advantageous to employ a non-genetic source for the transposase. We here show that a novel Cell-penetrating peptide, M918, has the ability to facilitate cellular delivery of both the transposase Sleeping Beauty as a protein and a transposon donor-plasmid carrying an antibiotic resistance gene in vitro. The technique is a simple and straightforward one-step method that might render a safe and efficient delivery platform for Sleeping Beauty mediated gene therapy.
Place, publisher, year, edition, pages
2008. Vol. 14, no 1, 58-63 p.
Cell-penetrating peptides, Protein delivery, Transfection, Transposons
Biological Sciences Chemical Sciences
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-20685DOI: 10.1007/s10989-007-9114-zISI: 000253571800009OAI: oai:DiVA.org:su-20685DiVA: diva2:187211