Protein delivery by the cell-penetrating peptide YTA2
2007 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 18, no 1, 170-174 p.Article in journal (Refereed) Published
In most cases, the transport of cell-penetrating peptide (CPP) with a cargo molecule over the plasma membrane requires a cross-linking of the cargo molecule to the peptide. Lately, a method of cargo delivery, coincubation with CPP, has been applied. We have studied uptake and toxicity of the CPP, YTA2, in the Bowes human melanoma cell line and human MDA-MB-231 breast cancer cell line and compared the results with known cell-penetrating peptides. The results show that fluoresceinyl YTA2 is taken up by the Bowes cells with 3.23 nmol/mg protein and shows low membrane toxicity to the cells with an EC50 of 60 μM. Furthermore, we show that YTA2 is capable of delivering cargo proteins, such as β-galactosidase and tetramethyl rhodamine iso-thiocyanate (TRITC) labeled streptavidin into cells by coincubation. The delivery of TRITC-labeled streptavidin was quantified to 42.4 pmol streptavidin/mg protein. The delivery of proteins into the cells by mere coincubation is an advantage, since the chemical coupling between the CPP and the cargo molecule, which adds time-consuming synthesis and purification steps, can be omitted. In addition, the flexibility in CPP cargo delivery is increased.
Place, publisher, year, edition, pages
2007. Vol. 18, no 1, 170-174 p.
Biological Sciences Chemical Sciences
IdentifiersURN: urn:nbn:se:su:diva-20830DOI: 10.1021/bc060266gISI: 000243503400022OAI: oai:DiVA.org:su-20830DiVA: diva2:187356