ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3-L1 and F442A cells.
2007 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 581, no 17, 3157-63 p.Article in journal (Refereed) Published
Elongation of very long-chain fatty acids (ELOVL) members were overexpressed in two preadipocyte cell lines, ELOVL2 and ELOVL3 in 3T3-L1 cells, and ELOVL1–3 in F442A cells. Cells overexpressing ELOVL2, whose preferred substrates are arachidonic acid (AA, C20:4n−6) and eicosapentaenoic acid (EPA, C20:5n−3), showed an enhanced triacylglycerol (TAG) synthesis and subsequent accumulation of lipid droplets. Incorporation of fatty acid (FA) but not of glucose into TAG was enhanced by ELOVL2-overexpression. Two lipogenic genes encoding diacylglycerol acyltransferase-2 (DGAT2) and fatty acid-binding protein-4 (FABP4, aP2) were induced in ELOVL2-overexpressing cells, whereas no such effect was seen on the fatty acid synthase (FAS) gene.
Place, publisher, year, edition, pages
Elsevier , 2007. Vol. 581, no 17, 3157-63 p.
3T3-L1 Cells, Adipocytes/*metabolism, Animals, Cells; Cultured, Fatty Acids/metabolism, Gene Expression Regulation, Glucose/metabolism, Lipid Metabolism/genetics, Membrane Proteins/*genetics/metabolism, Mice, NIH 3T3 Cells, Transfection, Triglycerides/*biosynthesis
IdentifiersURN: urn:nbn:se:su:diva-21012DOI: 10.1016/j.febslet.2007.05.081ISI: 000248145800005PubMedID: 17583696OAI: oai:DiVA.org:su-21012DiVA: diva2:187538