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Profilin and actin localizes to Synaptotagmin-induced filopodia
Stockholm University, Faculty of Science, Wenner-Gren Institute for Experimental Biology.
2007 (English)Other (Other (popular science, discussion, etc.))
Abstract [en]

Profilin and actin localizes to Synaptotagmin-induced filopodia

Anna-Karin Johnsson, Roger Karlsson

Department of Cell Biology, The Wenner-Gren Institute, Stockholm University, S-10691 Stockholm, Sweden.

We are interested in the dynamics of the microfilament system at the leading edge. Our main focus is to explore how profilin:actin is transported to actin polymer forming sites at the inner leaflet of the plasma membrane. As a model system to study this process we are overexpressing synaptotagmin 1 in mammalian cells. This leads to a dramatic change in cell morphology with numerous oversized filopodia beeing formed. In addition to using this effect as a model system we are also interested in characterizing the mechanism responsible for this process. We have seen that the synaptotagmin 1 induced filopodia stain positively for profilin and actin and that they are enriched in the phosphatidylinositol lipid PIP2. Through cotransfection with a dominant negative form of Cdc42 we have indications that the formation of these filopodia is dependent on this small Rho GTPase.

ASCB/ECF-möte i Dijon 27-30 juni 2007

Place, publisher, year, edition, pages
URN: urn:nbn:se:su:diva-22256OAI: diva2:188783
Available from: 2007-12-20 Created: 2007-12-20Bibliographically approved

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