Differential membrane perturbation caused by the cell penetrating peptide Tp10 depending on attached cargo
2007 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 581, no 13, 2389-2393 p.Article in journal (Refereed) Published
The membrane leakage caused by the cell penetrating peptide Tp10, a variant of transportan, was studied in large unilamellar vesicles with the entrapped fluorophore calcein. The vesicles were composed of zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. A significant decrease in membrane leakage was found when the 55 kDa streptavidin protein was attached to Tp10. When a 5.4 kDa peptide nucleic acid molecule was attached, the membrane leakage was comparable to that caused by Tp10 alone. The results suggest that direct membrane effects may cause membrane translocation of Tp10 alone and of smaller complexes, whereas these effects do not contribute for larger cargoes.
Place, publisher, year, edition, pages
2007. Vol. 581, no 13, 2389-2393 p.
cell-penetrating peptides, transportan 10, cell membrane perturbation, streptavidin, peptide nucleic acid
IdentifiersURN: urn:nbn:se:su:diva-22319DOI: 10.1016/j.febslet.2007.04.046ISI: 000247087600002PubMedID: 17485081OAI: oai:DiVA.org:su-22319DiVA: diva2:188846