Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. (E. Glaser)
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. (E. Glaser)
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. (E. Glaser)
2006 (English)In: Biol Chem, ISSN 1431-6730, Vol. 387, no 10-11, 1441-7 p.Article in journal (Refereed) Published
Abstract [en]

Two novel metalloendopeptidases in Arabidopsis thaliana, AtPreP1 and AtPreP2, are responsible for the degradation of targeting peptides in mitochondria and chloroplasts. Both AtPreP1 and AtPreP2 contain ambiguous targeting peptides and are dually targeted to both organelles. The proteases also have the capacity to degrade unstructured peptides of up to 65 amino acid residues, but not small proteins. The catalysis occurs in a huge catalytic chamber revealed by the crystal structure of AtPreP1 at 2.1 A. The enzymes show a preference for basic and small uncharged amino acids or serines at the cleavage sites. Despite similarities in cleavage specificities, cleavage-site recognition differs for both proteases and is context- and structure-dependent. The AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis.

Place, publisher, year, edition, pages
2006. Vol. 387, no 10-11, 1441-7 p.
Keyword [en]
Animals, Arabidopsis/*enzymology/genetics, Arabidopsis Proteins/genetics/*metabolism, Chloroplasts/*enzymology, Humans, Mitochondria/*enzymology, Peptide Hydrolases/genetics/*metabolism, Substrate Specificity
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:su:diva-22513PubMedID: 17081117OAI: oai:DiVA.org:su-22513DiVA: diva2:189040
Available from: 2007-12-21 Created: 2007-12-21 Last updated: 2011-01-11Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17081117&dopt=Citation

Search in DiVA

By author/editor
Glaser, ElzbietaNilsson, Stefan
By organisation
Department of Biochemistry and Biophysics
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 21 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf