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Expression, purification, crystallization and preliminary diffraction studies of the tRNA pseudouridine synthase TruD from Escherichia coli
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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2004 In: Acta Crystallographica Section D - Biological Crystallography, Vol. 60, 775-776 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2004. Vol. 60, 775-776 p.
URN: urn:nbn:se:su:diva-22731OAI: diva2:189335
Part of urn:nbn:se:su:diva-1060Available from: 2006-05-18 Created: 2006-05-18Bibliographically approved
In thesis
1. A structural genomics pilot project: methods and applications
Open this publication in new window or tab >>A structural genomics pilot project: methods and applications
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

With the availability of many completely sequenced genomes, scientific research has shifted from genes to the products of the genes, the proteins. Structural genomics groups have been established worldwide, with the objective of determining protein structures on a genome-wide scale. New methods for protein production and structural determination have become necessary.

Two methods for high throughput analysis of proteins are presented in the first part of this thesis. The first method is the thermofluor method, which presents a fast way to identify stabilizing conditions for a particular protein. It was shown that the addition of a stabilizing additive, identified with the thermofluor method, significantly increased the likelihood of growing protein crystals. The second method presented in this thesis provides a fast and robust way to detect metal containing proteins.

The second part of this thesis describes the crystal structures of two RNA modifying enzymes, the pseudouridine synthase TruD and the RNA m5C methyltransferase YebU. The catalytic domain of TruD was shown to bear remarkable structural similarity to the other pseudouridine families despite a lack of sequence similarity. In addition to the catalytic domain, the structure of TruD also contained an insertion domain with a novel fold.

YebU was also found have two structurally distinct domains. The N-terminal catalytic domain has a high structural similarity to other RNA m5C methyltransferases. The C-terminal domain was revealed to be a so-called PUA domain, which had not been predicted by previous sequence alignments.

Place, publisher, year, edition, pages
Stockholm: Institutionen för biokemi och biofysik, 2006. 71 p.
National Category
Other Industrial Biotechnology
urn:nbn:se:su:diva-1060 (URN)91-7155-283-9 (ISBN)
Public defence
2006-06-08, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 14:00
Available from: 2006-05-18 Created: 2006-05-18Bibliographically approved

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