Determination of the content and identity of lidocaine soluions with UV-visible spectroscopy and multivariate calibration
2001 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 126, no 7, 1142-1148 p.Article in journal (Refereed) Published
A method is proposed for the determination of the content and identity of the active compound in pharmaceutical solutions by means of ultraviolet-visible (UV–Vis) spectroscopy, orthogonal signal correction (OSC) and multivariate calibration with soft independent modelling of class analogy (SIMCA) classification and partial least squares (PLS) regression. The content was determined with PLS regression and the identity with PLS regression and SIMCA classification. The method was tested on the local anaesthetic compound lidocaine. For the validation, external test sets of both manufactured sample solutions and samples from a stability study were used. For comparison with this new method, liquid chromatography was used as a reference method. The results show that in respect of accuracy, precision and repeatability, the new method is comparable to the reference method. The main advantage over liquid chromatography is the much shorter time of analysis and the simpler analytical procedure. An estimate of the analysis time saved with the proposed method compared with using liquid chromatography, together with practical considerations, is given.
Place, publisher, year, edition, pages
2001. Vol. 126, no 7, 1142-1148 p.
IdentifiersURN: urn:nbn:se:su:diva-22773DOI: 10.1039/b102545gOAI: oai:DiVA.org:su-22773DiVA: diva2:189418
Part of urn:nbn:se:su:diva-1102004-04-222004-04-222010-08-02Bibliographically approved