Insulin-like growth factor type 1 up-regulates uncoupling protein 3
2001 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 287, no 5, 1105-1111 p.Article in journal (Refereed) Published
In this study the expression of uncoupling protein 3 (UCP3) and its regulation by insulin-like growth factor 1 (IGF-I) and insulin in human neuroblastoma SH-SY5Y cells were characterized. Reverse transcriptase-PCR, Western blot, and immunofluorescence analysis showed that SH-SY5Y cells express UCP3 natively. IGF-I induced a time- and concentration-dependent induction of UCP3 protein reaching a twofold expression after 72 h with 10 nM IGF-I. Extremely high insulin concentrations (860 nM) and 10 nM trIGF-I, a truncated form of IGF-I with the same affinity for the IGF-I receptor as the full-length IGF-I, but with lower activity on the insulin receptor, also upregulated UCP3. We conclude that SH-SY5Y cells express UCP3 natively and that the expression is regulated by IGF-I via the IGF-I receptor.
Place, publisher, year, edition, pages
2001. Vol. 287, no 5, 1105-1111 p.
uncoupling protein, IGF-I, SH-SY5Y, insulin, diabetes
IdentifiersURN: urn:nbn:se:su:diva-22832DOI: 10.1006/bbrc.2001.5702OAI: oai:DiVA.org:su-22832DiVA: diva2:189593
Part of urn:nbn:se:su:diva-1212004-04-282004-04-282015-03-09Bibliographically approved