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Proton, Electron, and O2 transfer in Cytochrome c Oxidase
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In mitochondria and aerobic bacteria energy conservation involves electron transfer through the membrane-bound protein of the respiratory chain to oxygen. Cytochrome c oxidase (CcO) catalyzes the reduction of oxygen to water. Part of the energy released in this reaction is used to pump protons across the membrane. The protons that are pumped, and those needed for reduction to water, are taken from the inside of the membrane. This helps to maintain an electrochemical potential over the membrane, which is necessary for the survival of the cell and is used for example to produce ATP.

This work has been focused on the proton, electron and oxygen transfer in CcO from Rhodobacter sphaeroides. Results presented here show that there is a need for a specific O2-channel, due to structural rigidity of the area close to the catalytic site. In other parts of the enzyme regions that undergo redox-dependent structural changes were identified, and we proposed areas involved in controlling proton pumping and in forming the proton exit route.

The results from investigating vectorial proton translocation defined the order and timing of proton uptake and release during a pumping-cycle. Indications from these results also suggested a mechanism by which CcO couples electron transfer and proton pumping, assuring that electrons are not transferred without simultaneous proton pumping.

Place, publisher, year, edition, pages
Stockholm: Institutionen för biokemi och biofysik , 2006. , 150 p.
Keyword [en]
cytochrome c oxidase, electron transfer, proton transfer, proton pumping, kinetic isotope effect
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-1222ISBN: 91-7155-302-9 (print)OAI: oai:DiVA.org:su-1222DiVA: diva2:189624
Public defence
2006-10-06, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 10:00
Opponent
Supervisors
Available from: 2006-09-14 Created: 2006-09-14Bibliographically approved
List of papers
1. A single-amino-acid lid renders a gas-tight compartment within a membrane-bound transporter
Open this publication in new window or tab >>A single-amino-acid lid renders a gas-tight compartment within a membrane-bound transporter
2004 In: Proc. Natl. Acad. Sci. USA, Vol. 101, no 32, 11617-11621 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-22844 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14Bibliographically approved
2. Mapping protein dynamics in catalytic intermediates of the redox-driven proton pump cyrochrome c oxidase.
Open this publication in new window or tab >>Mapping protein dynamics in catalytic intermediates of the redox-driven proton pump cyrochrome c oxidase.
In: Proc. Natl. Acad. Sci. USAArticle in journal (Refereed) Submitted
Identifiers
urn:nbn:se:su:diva-22845 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14Bibliographically approved
3. The timing of proton migration in membrane-reconstituted cytochrome c oxidase
Open this publication in new window or tab >>The timing of proton migration in membrane-reconstituted cytochrome c oxidase
2005 In: Proc. Natl. Acad. Sci. USA, Vol. 102, no 49, 17624-17629 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-22846 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14Bibliographically approved
4. Structural elements involved i internal proton transfer reactions in cytochrome c oxidase
Open this publication in new window or tab >>Structural elements involved i internal proton transfer reactions in cytochrome c oxidase
Manuscript (Other academic)
Identifiers
urn:nbn:se:su:diva-22847 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14 Last updated: 2010-01-13Bibliographically approved
5. The proton exit route of cytochrome c oxidase
Open this publication in new window or tab >>The proton exit route of cytochrome c oxidase
Show others...
Manuscript (Other academic)
Identifiers
urn:nbn:se:su:diva-22848 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14 Last updated: 2010-01-13Bibliographically approved
6. Inhibition of proton pumping by zinc ions during specific reaction steps in cytochrome c oxidase
Open this publication in new window or tab >>Inhibition of proton pumping by zinc ions during specific reaction steps in cytochrome c oxidase
2006 In: Biochim. Biophys. Acta, Vol. 1757, 388-394 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-22849 (URN)
Note
Part of urn:nbn:se:su:diva-1222Available from: 2006-09-14 Created: 2006-09-14Bibliographically approved

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