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The Prx Q protein of Arabidopsis thaliana is a member of the luminal chloroplast proteome
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2006 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 580, no 26, 6055-6061 p.Article in journal (Refereed) Published
Abstract [en]

Peroxiredoxins have been discovered in many organisms ranging from eubacteria to mammals, and their known biological functions include both oxidant defense and signal transduction. The genome of Arabidopsis thaliana encodes for ten individual peroxiredoxins, of which four are located in the chloroplast. The best-characterized member of the chloroplast peroxiredoxins is 2-Cys Prx that is associated with the stroma side of the thylakoid membrane and is considered to participate in antioxidant defense and protection of photosynthesis. This study addressed the chloroplast peroxiredoxin Prx Q and showed that its subcellular location is the lumen of the thylakoid membrane. To get insight in the biological function of the Prx Q protein of Arabidopsis, the protein levels of the Prx Q protein in thylakoid membranes were studied under different light conditions and oxidative stress. A T-DNA knockout mutant of Prx Q did not show any visible phenotype and had normal photosynthetic performance with a slightly increased oxygen evolving activity.

Place, publisher, year, edition, pages
2006. Vol. 580, no 26, 6055-6061 p.
Keyword [en]
Photosynthesis, PSII, Peroxiredoxin
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
URN: urn:nbn:se:su:diva-22992DOI: 10.1016/j.febslet.2006.10.001OAI: diva2:189866
Part of urn:nbn:se:su:diva-1319Available from: 2006-11-01 Created: 2006-11-01 Last updated: 2010-08-05Bibliographically approved
In thesis
1. Overview and characterisation of the thylakoid lumen of higher plants
Open this publication in new window or tab >>Overview and characterisation of the thylakoid lumen of higher plants
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In higher plants, chloroplasts are the site for the photosynthetic reactions, converting solar energy to chemical energy. Within the chloroplast the thylakoid membrane network encloses the soluble lumen compartment. Until recently the knowledge of the lumen composition and function was limited, but a more profound understanding of the thylakoid lumen content is gradually emerging. The discovery that the thylakoid lumen contains numerous enzymes, besides the already known proteins directly involved or associated with the photosynthetic reactions, have changed the view on this compartment.

The first part of the thesis the lumen proteome maps of Arabidopsis and spinach were resolved. These two proteome maps showed good correlation and the same protein groups were represented in the two proteomes. Thirty eight proteins were identified and in combination with an in silico prediction for the proteome it was estimated that at least 80 different proteins are lumen located.

The second part was to further investigate the functions of two lumen localized proteins, the cyclophilin AtCYP20-2 and peroxiredoxin Q (PrxQ). AtCYP20-2 is suggested to be responsible for the major peptidyl prolyl cis/trans isomerases (PPIase) activity in the lumen. In AtCYP20-2 knockout mutants the total PPIase activity is not altered, instead AtFKBP13 may be oxidatively activated to compensate for the loss of AtCYP20-2. With respect to the PrxQ protein we were able to show that it is a lumenal protein that seems to exist as a soluble pool in the lumen as no interaction with the thylakoid membrane could be detected.

In the last part of this thesis the dynamics of the thylakoid membrane and lumen proteome were studied during oxidative stress. Important changes to the proteome were observed in response to the stress treatment, including abundance changes of proteins related to photosynthesis, as well as proteins not previously identified in relation to stress.

Place, publisher, year, edition, pages
Stockholm: Institutionen för biokemi och biofysik, 2006. 51 p.
Arablidopsis, thylakoid lumen, AtCYP20-2, Peroxiredoxin Q, oxidative stress
National Category
Biochemistry and Molecular Biology
urn:nbn:se:su:diva-1319 (URN)91-7155-299-5 (ISBN)
Public defence
2006-11-24, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 10:00
Available from: 2006-11-01 Created: 2006-11-01Bibliographically approved

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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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