Identification and evolution of dual-topology membrane proteins
2006 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 13, no 2, 112-116 p.Article in journal (Refereed) Published
Integral membrane proteins are generally believed to have unique membrane topologies. However, it has been suggested that dual-topology proteins that adopt a mixture of two opposite orientations in the membrane may exist. Here we show that the membrane orientations of five dual-topology candidates identified in Escherichia coli are highly sensitive to changes in the distribution of positively charged residues, that genes in families containing dual-topology candidates occur in genomes either as pairs or as singletons and that gene pairs encode two oppositely oriented proteins whereas singletons encode dual-topology candidates. Our results provide strong support for the existence of dual-topology proteins and shed new light on the evolution of membrane-protein topology and structure.
Place, publisher, year, edition, pages
2006. Vol. 13, no 2, 112-116 p.
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-23012DOI: 10.1038/nsmb1057OAI: oai:DiVA.org:su-23012DiVA: diva2:189899