Emulating membrane protein evolution by rational design
2007 (English)In: Science, ISSN 0036-8075, Vol. 315, no 5816, 1282-1284 p.Article in journal (Refereed) Published
How do integral membrane proteins evolve in size and complexity? Using the small multidrug-resistance protein EmrE from Escherichia coli as a model, we experimentally demonstrated that the evolution of membrane proteins composed of two homologous but oppositely oriented domains can occur in a small number of steps: An original dual-topology protein evolves, through a gene-duplication event, to a heterodimer formed by two oppositely oriented monomers. This simple evolutionary pathway can explain the frequent occurrence of membrane proteins with an internal pseudo–two-fold symmetry axis in the plane of the membrane.
Place, publisher, year, edition, pages
2007. Vol. 315, no 5816, 1282-1284 p.
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-23013DOI: 10.1126/science.1135406ISI: 000244564700051OAI: oai:DiVA.org:su-23013DiVA: diva2:189900