A crucial role for profilin-actin in the intracellular motility of Listeria monocytogenes
2003 (English)In: EMBO reports, ISSN 1469-221, Vol. 4, no 5, 523-529 p.Article in journal (Refereed) Published
We have examined the effect of covalently crosslinked profilin–actin (PxA), which closely matches the biochemical properties of ordinary profilin–actin and interferes with actin polymerization in vitro and in vivo, on Listeria monocytogenes motility. PxA caused a marked reduction in bacterial motility, which was accompanied by the detachment of bacterial tails. The effect of PxA was dependent on its binding to proline-rich sequences, as shown by the inability of PH133SxA, which cannot interact with such sequences, to impair Listeria motility. PxA did not alter the motility of a Listeria mutant that is unable to recruit Ena (Enabled)/VASP (vasodilator-stimulated phosphoprotein) proteins and profilin to its surface. Finally, PxA did not block the initiation of actin-tail formation, indicating that profilin–actin is only required for the elongation of actin filaments at the bacterial surface. Our findings provide further evidence that profilin–actin is important for actin-based processes, and show that it has a key function in Listeria motility.
Place, publisher, year, edition, pages
London: Nature Publishing Group , 2003. Vol. 4, no 5, 523-529 p.
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-23150DOI: 10.1038/sj.embor.embor823OAI: oai:DiVA.org:su-23150DiVA: diva2:190412
Part of urn:nbn:se:su:diva-1682004-05-132004-05-132009-12-22Bibliographically approved