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Structural and immunochemical relationship between the O-antegenic polysaccharides from the enteroaggregative Escherichia coli strain 396/C1 and Escherichia coli O126
Stockholm University, Faculty of Science, Department of Organic Chemistry.
bKarolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Karolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology.
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2004 (English)In: Carbohydrate Research, ISSN 0008-6215, Vol. 339, no 8, 1491-1496 p.Article in journal (Refereed) Published
Abstract [en]

The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 396/C-1 has been determined. Sugar and methylation analyses together with 1H and 13C NMR spectroscopy were the main methods used. Inter-residue correlations were determined by 1H,1H-NOESY, 1H,13C-heteronuclear multiple-bond correlation and dipole–dipole cross-correlated relaxation experiments. The PS is composed of pentasaccharide repeating units with the following structure:

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Analysis of NMR data reveals that on average the PS consists of 13 repeating units and indicates that the biological repeating unit contains an N-acetylglucosamine residue at its reducing end. This structure is different to that reported for the O-antigen polysaccharide from E. coli O126. Monospecific anti-E. coli O126 rabbit serum from The International Escherichia and Klebsiella Centre did not distinguish between the E. coli strain 396/C-1 and the E. coli O126 reference strain, neither in slide agglutination nor in an indirect enzyme immunoassay. Subsequent successful serotyping of the E. coli strain 396/C-1 showed it to be E. coli O126:K+:H27.

Place, publisher, year, edition, pages
Elsevier , 2004. Vol. 339, no 8, 1491-1496 p.
Keyword [en]
Escherichia coli; Enteroaggregative; Serology; Lipopolysaccharide; NMR
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-23167DOI: 10.1016/j.carres.2004.04.002OAI: oai:DiVA.org:su-23167DiVA: diva2:190474
Note
Part of urn:nbn:se:su:diva-172Available from: 2004-05-13 Created: 2004-05-13 Last updated: 2009-12-28Bibliographically approved
In thesis
1. Synthesis, structure and conformation of oligo- and polysaccharides
Open this publication in new window or tab >>Synthesis, structure and conformation of oligo- and polysaccharides
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Carbohydrates are a complex group of biomolecules with a high structural diversity. Their almost omnipresent occurrence has generated a broad field of research in both biology and chemistry. This thesis focuses on three different aspects of carbohydrate chemistry, synthesis, structure elucidation and the conformational analysis of carbohydrates.

The first paper describes the synthesis of a penta- and a tetrasaccharide related to the highly branched capsular polysaccharide from Streptococcus pneumoniae type 37. In the second paper, the structure of the O-antigenic repeating unit from the lipopolysaccharide of E. coli 396/C1 was determined along with indications of the structure of the biological repeating unit. In addition, its structural and immunological relationship with E. coli O126 is discussed. In the third paper, partially protected galactopyranosides were examined to clarify the origin of an intriguing 4JHO,H coupling, where a W-mediated coupling pathway was found to operate. In the fourth paper, the conformation of methyl a-cellobioside is studied with a combination of molecular dynamics simulations and NMR spectroscopy. In addition to the expected syn-conformation, detection and quantification of anti-ø and anti-ψ conformers was also possible.

Place, publisher, year, edition, pages
Stockholm: Institutionen för organisk kemi, 2004. 40 p.
National Category
Organic Chemistry
Identifiers
urn:nbn:se:su:diva-172 (URN)91-7265-872-X (ISBN)
Public defence
2004-06-03, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 14:00
Opponent
Supervisors
Available from: 2004-05-13 Created: 2004-05-13Bibliographically approved

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