Structural and immunochemical relationship between the O-antegenic polysaccharides from the enteroaggregative Escherichia coli strain 396/C1 and Escherichia coli O126
2004 (English)In: Carbohydrate Research, ISSN 0008-6215, Vol. 339, no 8, 1491-1496 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 396/C-1 has been determined. Sugar and methylation analyses together with 1H and 13C NMR spectroscopy were the main methods used. Inter-residue correlations were determined by 1H,1H-NOESY, 1H,13C-heteronuclear multiple-bond correlation and dipole–dipole cross-correlated relaxation experiments. The PS is composed of pentasaccharide repeating units with the following structure:
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Analysis of NMR data reveals that on average the PS consists of 13 repeating units and indicates that the biological repeating unit contains an N-acetylglucosamine residue at its reducing end. This structure is different to that reported for the O-antigen polysaccharide from E. coli O126. Monospecific anti-E. coli O126 rabbit serum from The International Escherichia and Klebsiella Centre did not distinguish between the E. coli strain 396/C-1 and the E. coli O126 reference strain, neither in slide agglutination nor in an indirect enzyme immunoassay. Subsequent successful serotyping of the E. coli strain 396/C-1 showed it to be E. coli O126:K+:H27.
Place, publisher, year, edition, pages
Elsevier , 2004. Vol. 339, no 8, 1491-1496 p.
Escherichia coli; Enteroaggregative; Serology; Lipopolysaccharide; NMR
IdentifiersURN: urn:nbn:se:su:diva-23167DOI: 10.1016/j.carres.2004.04.002OAI: oai:DiVA.org:su-23167DiVA: diva2:190474
Part of urn:nbn:se:su:diva-1722004-05-132004-05-132009-12-28Bibliographically approved