DNA Repair Rate and Etoposide (VP16) Resistance of Tumor Cell Subpopulations derived from a Single Human Small Cell Lung Cancer
2003 (English)In: Lung Cancer, ISSN 0169-5002, Vol. 40, no 2, 157-64 p.Article in journal (Refereed) Published
Two human small cell lung cancer (SCLC) subpopulations, CPH 54A, and CPH 54B, established from the same patient tumor by in vitro cloning, were investigated. The tumor was classified as intermediate-type SCLC. The cellular sensitivity to ionizing radiation (IR) was previously determined in the two sublines both in vivo and in vitro. Here we measured the etoposide (VP16) sensitivity together with the induction and repair of VP16- and IR-induced DNA double-strand breaks (DSBs). The two subpopulations were found to differ significantly in sensitivity to VP16, with the radioresistant 54B subline also being VP16 resistant. In order to explain the VP16 resistant phenotype several mechanisms where considered. The p53 status, P-glycoprotein, MRP, topoisomerase IIα, and Mre11 protein levels, as well as growth kinetics, provided no explanations of the observed VP16 resistance. In contrast, a significant difference in repair of both VP16- and IR-induced DSBs, together with a difference in the levels of the DSB repair proteins DNA-dependent protein kinase (DNA-PKcs) and RAD51 was observed. The VP16- and radioresistant 54B subline exhibited a pronounced higher repair rate of DSBs and higher protein levels of both DNA-PKcs and RAD51 compared with the sensitive 54A subline. We suggest, that different DSB repair rates among tumor cell subpopulations of individual SCLC tumors may be a major determinant for the variation in clinical treatment effect observed in human SCLC tumors of identical histological subtype.
Place, publisher, year, edition, pages
Elsevier , 2003. Vol. 40, no 2, 157-64 p.
DNA-PKcs; RAD51; DNA double-strand break repair; VP16; Etoposide; Ionizing radiation; Drug resistance; Small cell lung cancer
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-23260DOI: 10.1016/S0169-5002(03)00026-6OAI: oai:DiVA.org:su-23260DiVA: diva2:190949
Part of urn:nbn:se:su:diva-2072004-08-202004-08-202010-01-07Bibliographically approved