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Molecular characterization, immunological and phylogenetic analysis of Hemolin, in the Chinese oak silkmoth, Antheraea pernyi
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
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Manuscript (Other academic)
Identifiers
URN: urn:nbn:se:su:diva-23284OAI: oai:DiVA.org:su-23284DiVA: diva2:191157
Note
Part of urn:nbn:se:su:diva-224Available from: 2004-09-02 Created: 2004-09-02 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Anti-parasitic and anti-viral immune responses in insects
Open this publication in new window or tab >>Anti-parasitic and anti-viral immune responses in insects
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Insects encounter many microorganisms in nature and to survive they have developed counter measures against the invading pathogens. In Drosophila melanogaster research on insect immunity has mainly been focused on infections by bacteria and fungi. We have explored the immune response against natural infections of the parasite Octosporea muscaedomesticae and the Drosophila C virus as compared to natural infections of bacteria and fungi. By using Affymetrix Drosophila GeneChips, we were able to obtain 48 genes uniquely induced after parasitic infection. It was also clearly shown that natural infections led to different results than when injecting the pathogens.

In order to search for the ultimate role of the lepidopteran protein hemolin, we used RNA interference (RNAi). We could show that injection of double stranded RNA (dsRNA) of Hemolin in pupae of Hyalophora cecropia led to embryonic malformation and lethality and that there was a sex specific difference. We continued the RNAi investigation of hemolin in another lepidopteran species, Antheraea pernyi, and discovered that hemolin was induced by dsRNA per se. A similar induction of hemolin was seen after infection with baculovirus and we therefore performed in vivo experiments on baculovirus infected pupae. We could show that a low dose of dsHemolin prolonged the period before the A. pernyi pupae showed any symptoms of infection, while a high dose led to a more rapid onset of symptoms. By performing in silico analysis of the hemolin sequence from A. pernyi in comparison with other Hemolin sequences, it was possible to select a number of sites that either by being strongly conserved or variable could be important targets for future studies of hemolin function.

Place, publisher, year, edition, pages
Stockholm: Institutionen för genetik, mikrobiologi och toxikologi, 2004. 68 p.
Keyword
Hemolin, Hyalophora cecropia, Antheraea pernyi, Drosophila melanogaster, anti-viral, anti-parasitic, rna interference, microarray, innate immunity
National Category
Genetics
Identifiers
urn:nbn:se:su:diva-224 (URN)91-7265-930-0 (ISBN)
Public defence
2004-09-24, sal G, Arrheniuslaboratorierna, Svante Arrhenius väg 14-18, Stockholm, 13:00
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Available from: 2004-09-02 Created: 2004-09-02Bibliographically approved

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