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Structural studies of an exopolysaccharide produced by Streptococcus thermophilus THS
Stockholm University, Faculty of Science, Department of Organic Chemistry.
2005 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 6, no 1, 105-108 p.Article in journal (Refereed) Published
Abstract [en]

The structure of an extracellular polysaccharide (EPS) from Streptococcus thermophilus THS has been determined. A combination of component analysis, methylation analysis and NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by two-dimensional 1H,1H−NOESY and 1H,13C−HMBC NMR experiments. NMR data indicate different mobility within the EPS with a stiffer backbone and a more flexible side-chain.

Place, publisher, year, edition, pages
2005. Vol. 6, no 1, 105-108 p.
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-23398DOI: 10.1021/bm0496514OAI: oai:DiVA.org:su-23398DiVA: diva2:191931
Available from: 2004-11-11 Created: 2004-11-11 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Structural and Interaction Studies of Bacterial Polysaccharides by NMR Spectroscopy
Open this publication in new window or tab >>Structural and Interaction Studies of Bacterial Polysaccharides by NMR Spectroscopy
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

An introduction to bacterial polysaccharides and the methods for structural determination are described in the first two parts of the thesis.

In a structural elucidation of bacterial polysaccharides NMR experiments are important as is component analysis. A short description of immunochemical methods such as enzyme immunoassays is included. Two NMR techniques used for interaction studies, trNOE and STD NMR, are also discussed.

The third part of the thesis discusses and summarizes the results from the included papers. The structures of the exopolysaccharides produced by two lactic acid bacteria are determined by one- and two dimensional NMR experiments. One is a heteropolysaccharide produced by Streptococcus thermophilus and the other a homopolysaccharide produced by Propionibacterium freudenreichii. The structure of an acidic polysaccharide from a marine bacterium with two serine residues in the repeating unit is also investigated. The structural and immunological relationship between two O-antigenic polysaccharides from Escherichia coli strain 180/C3 and O5 is discussed and investigated. Finally, interaction studies of an octasaccharide derived from the Salmonella enteritidis O-antigen and a bacteriophage are described which were performed with NMR experiments.

Place, publisher, year, edition, pages
Stockholm: Institutionen för organisk kemi, 2004. 50 p.
Keyword
bacterial polysaccharides, NMR, interaction studies, structural studies, Escherichia coli, Salmonella enteritidis, Streptococcus thermophilus, Propionibacterium freudenreichii, Pseudoalteromonas aliena, P22 TSP
National Category
Organic Chemistry
Identifiers
urn:nbn:se:su:diva-284 (URN)91-7265-971-8 (ISBN)
Public defence
2004-12-03, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 14:00
Opponent
Supervisors
Available from: 2004-11-11 Created: 2004-11-11Bibliographically approved

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