Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Sperm motility in Gasterosteiform fishes: The role of salinity and ovarian fluid
Stockholm University, Faculty of Science, Department of Zoology.
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In externally fertilising fishes, various factors in the surrounding environment may influence the viability of the sperm and eggs, thus determining the success of reproduction. In this thesis, the influence of salinity and ovarian fluid on sperm motility has been investigated in three Gasterosteiform fishes.

The three-spined stickleback, Gasterosteus aculeatus, has successfully invaded fresh water and differs from most other fishes in its ability to spawn in waters of all salinities. Our results show that sperm of the three-spined is strongly stimulated by the ovarian fluid surrounding the eggs. In fresh water, where the period of motility is only about a minute, ovarian fluid prolongs motility to last several hours. We show that this effect is due to fluid’s ionic content and that the fluid remains in the nest, surrounding the eggs, for a prolonged time due to the proteins and/or other macromolecules in the fluid. Our results explain how successful spawning can occur in fresh water despite that it takes several minutes for all three-spined stickleback eggs to be fertilised. The stimulating effect of ovarian fluid may be one of the factors that have enabled the originally marine three-spined stickleback to colonise fresh water.

The fifteen-spined stickleback, Spinachia spinachia, is exclusively marine. We found their sperm motility to be good in seawater, reduced in brackish water, and non-existent in freshwater. The presence of ovarian fluid made no difference in any salinity, a factor that might have contributed to their inability to colonise fresh water. Being regarded as a primitive member of the stickleback family, the lack of response to ovarian fluid suggests that this is not a primitive trait among the sticklebacks.

The male straight-nose pipefish, Nerophis ophidion carries the eggs attached to its ventral surface. Fertilisation has previously been suggested to be external, but our results show that their sperm are not motile in seawater alone, but in a mixture of seawater and ovarian fluid. This result, together with the finding of some sperm in the female genital area, and that the sperm head is elongated, suggests that the fertilisation in the straight-nose pipefish occurs in close proximity with the eggs and ovarian fluid. This could then explain why the straight-nose pipefish has minute testes and complete confidence of paternity.

Place, publisher, year, edition, pages
Stockholm: Zoologiska institutionen , 2005.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-381ISBN: 91-7155-021-6 (print)OAI: oai:DiVA.org:su-381DiVA: diva2:193185
Public defence
2005-03-18, sal G, Arrheniuslaboratorierna, Svante Arrhenius väg 14-18, Stockholm, 10:00
Opponent
Supervisors
Available from: 2005-02-24 Created: 2005-02-24Bibliographically approved
List of papers
1. Long lasting stickleback sperm: is ovarian fluid a key to success in fresh water?
Open this publication in new window or tab >>Long lasting stickleback sperm: is ovarian fluid a key to success in fresh water?
Show others...
2003 In: Journal of Fish Biology, ISSN 0022-1112, Vol. 63, no 1, 240-253 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-23595 (URN)
Note
Part of urn:nbn:se:su:diva-381Available from: 2005-02-24 Created: 2005-02-24Bibliographically approved
2. Influence of salinity and ovarian fluid on sperm motility in the fifteenspined stickleback
Open this publication in new window or tab >>Influence of salinity and ovarian fluid on sperm motility in the fifteenspined stickleback
2003 In: Journal of Fish Biology, ISSN 0022-1112, Vol. 63, 1429-1438 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-23596 (URN)
Note
Part of urn:nbn:se:su:diva-381Available from: 2005-02-24 Created: 2005-02-24Bibliographically approved
3. Stickleback sperm saved by salt in ovarian fluid
Open this publication in new window or tab >>Stickleback sperm saved by salt in ovarian fluid
Manuscript (Other academic)
Identifiers
urn:nbn:se:su:diva-23597 (URN)
Note
Part of urn:nbn:se:su:diva-381Available from: 2005-02-24 Created: 2005-02-24 Last updated: 2010-01-13Bibliographically approved
4. Why no sperm competition in pipefish with externally brooding males?
Open this publication in new window or tab >>Why no sperm competition in pipefish with externally brooding males?
Show others...
Manuscript (Other academic)
Identifiers
urn:nbn:se:su:diva-23598 (URN)
Note
Part of urn:nbn:se:su:diva-381Available from: 2005-02-24 Created: 2005-02-24 Last updated: 2010-01-13Bibliographically approved

Open Access in DiVA

fulltext(361 kB)2820 downloads
File information
File name FULLTEXT01.pdfFile size 361 kBChecksum SHA-1
86c71d4f3b6c3bab78fe909569fec474ece9622a2093437e75b2574bd88b9cac78a85887
Type fulltextMimetype application/pdf

By organisation
Department of Zoology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 2820 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 886 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf