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Heat pretreatment increase cadmium resistance and HSP 70 levels in Baltic Sea mussels
Stockholm University, Faculty of Science, Department of Systems Ecology.
Stockholm University, Faculty of Science, Department of Systems Ecology.
Stockholm University, Faculty of Science, Department of Systems Ecology.
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2000 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 48, no 1, 1-12 p.Article in journal (Refereed) Published
Abstract [en]

The effects of heat treatment and cadmium exposure on the synthesis of a major stress inducible protein (hsp 70) and on the metabolism of the blue mussel Mytilus edulis L. from the Baltic Sea, were studied in a laboratory experiment. The mussels were kept in sea water of ambient salinity (6.3‰) and temperature (4°C). The effects of cadmium (20 μg l−1), measured as changes in physiological rates (oxygen consumption, ammonia excretion, clearance rates and scope for growth) and hsp 70 expression were studied at 4°C and in combination with a rapid rise in temperature to 20°C. Relatively low levels of hsp 70 were detected but the negative effect was reflected in a reduction of scope for growth of the exposed mussels compared to controls. This effect was more pronounced at 20°C. Mussels not exposed to cadmium in the first experiment were used in a second set of experiments. Heat shocked mussels were allowed to reacclimatise to 4°C for 5 days and then, along with the mussels already at 4°C, exposed to cadmium (20 μg l−1). The results clearly indicated that the mussels exposed to 20°C in the first experiment more rapidly induced synthesis of hsp 70 after cadmium exposure in the second experiment. Also the reacclimatised mussels exposed to heat shock but not to cadmium in the first experiment, induced some hsp 70 in the second experiment. This suggests that the rate of induction of heat shock or stress proteins in Baltic mussels is slower than what has been described for mussels from more marine environments. The mussels kept at 4°C throughout the experiment and exposed to cadmium showed low levels of hsp 70, again indicating a low rate of induction. The increasing levels of hsp 70 correlated well with a maintained level of physiological fitness, in terms of scope for growth, although the mussels showed increasing body burdens of cadmium.

Place, publisher, year, edition, pages
2000. Vol. 48, no 1, 1-12 p.
Keyword [en]
Stress protein; hsp 70; Mytilus; Baltic Sea; Brackish water; Stress; Cadmium
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:su:diva-23900DOI: 10.1016/S0166-445X(99)00030-2OAI: oai:DiVA.org:su-23900DiVA: diva2:195243
Note
Part of urn:nbn:se:su:diva-542Available from: 2005-05-13 Created: 2005-05-13 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Protein Expression in Baltic Sea Blue Mussels Exposed to Natural and Anthropogenic Stress: The use of stress inducible proteins in ecotoxicological studies
Open this publication in new window or tab >>Protein Expression in Baltic Sea Blue Mussels Exposed to Natural and Anthropogenic Stress: The use of stress inducible proteins in ecotoxicological studies
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The focus of this thesis is the early detection of stress in the environment. It has been proposed that studies on the cellular level would detect stress reactions earlier in time compared to common physiological methods. In a series of experiments we investigated how different stress factors, both natural and introduced by man, affect levels of stress proteins. One- and two-dimensional gels were used to determine individual proteins and families of proteins. The two-dimensional gels were also used in a proteomic approach, were the presence and absence of proteins after treatment was observed, and the protein expression signatures (PES) were identified.

Baltic Mytilus edulis was used in all experiments and it is evident that earlier observed differences in physiological rates and pollution sensitivity, compared to marine mussels, is also manifested as lower concentrations of stress proteins after exposure to copper and cadmium. When the Baltic mussels were allowed to acclimate for one month the difference decreased, suggesting an environmentally induced difference (paper I). Pre-exposure to heat before exposure to either a second heat-shock or cadmium was found to enhance the levels of HSP70 and thus tolerance, significantly (paper II). Exposure to a mixture of stress factors (PCB, copper and lowered salinity) revealed synergistic, additive and antagonistic effects in induction of 6 different stress proteins. When analyzing a large number of proteins it was shown that it is possible to identify PES with this technique, and we hypothesize that it could be possible to separate responses to mixtures of stress factors (Papers III and IV). Different techniques were also applied to analyze the protein expression pattern when mussels were exposed to PAH- and PCB-fractions extracted from Baltic Sea sediments. In this experiment the protein assays were accompanied by physiological measurements. All methods indicated stressed conditions, but the variation between individual mussels within treatments was smaller in terms of protein response than for physiological parameters (paper V). It is concluded that measuring the induction of stress proteins is a reliable way to detect stressful conditions. Proteins visualized on a one dimensional gel give a “gross” picture of an organism’s condition. The major challenge is to identify the origin and severity of the elucidated stress response. Further mapping of two-dimensional gels suggested that protein patterns are specific to type and level of stress.

A most important future step is to establish links between sub-cellular protein response to well known physiological effects. This should include long term experiments where altered protein expression signatures are linked to life history characteristics like survival, growth and reproductive success.

Place, publisher, year, edition, pages
Stockholm: Institutionen för systemekologi, 2005. 31 p.
National Category
Ecology
Identifiers
urn:nbn:se:su:diva-542 (URN)91-7155-087-9 (ISBN)
Public defence
2005-06-03, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 8 A, Stockholm, 13:00
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Available from: 2005-05-13 Created: 2005-05-13Bibliographically approved

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