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Advances in Separation Science: . Molecular Imprinting: Development of Spherical Beads and Optimization of the Formulation by Chemometrics.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

An intrinsic mathematical model for simulation of fixed bed chromatography was demonstrated and compared to more simplified models. The former model was shown to describe variations in the physical, kinetic, and operating parameters better than the latter ones. This resulted in a more reliable prediction of the chromatography process as well as a better understanding of the underlying mechanisms responsible for the separation. A procedure based on frontal liquid chromatography and a detailed mathematical model was developed to determine effective diffusion coefficients of proteins in chromatographic gels. The procedure was applied to lysozyme, bovine serum albumin, and immunoglobulin γ in Sepharose™ CL-4B. The effective diffusion coefficients were comparable to those determined by other methods.

Molecularly imprinted polymers (MIPs) are traditionally prepared as irregular particles by grinding monoliths. In this thesis, a suspension polymerization providing spherical MIP beads is presented. Droplets of pre-polymerization solution were formed in mineral oil with no need of stabilizers by vigorous stirring. The droplets were transformed into solid spherical beads by free-radical polymerization. The method is fast and the performance of the beads comparable to that of irregular particles. Optimizing a MIP formulation requires a large number of experiments since the possible combinations of the components are huge. To facilitate the optimization, chemometrics was applied. The amounts of monomer, cross-linker, and porogen were chosen as the factors in the model. Multivariate data analysis indicated the influence of the factors on the binding and an optimized MIP composition was identified. The combined use of the suspension polymerization method to produce spherical beads with the application of chemometrics was shown in this thesis to drastically reduce the number of experiments and the time needed to design and optimize a new MIP.

Place, publisher, year, edition, pages
Stockholm: Institutionen för analytisk kemi , 2007. , 158 p.
Keyword [en]
Chromatography, Parameter estimation, Diffusion, Molecularly imprinted polymer, Suspension polymerization, Chemometrics
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-6582ISBN: 978-91-631-8317-1 (print)OAI: oai:DiVA.org:su-6582DiVA: diva2:196688
Public defence
2007-02-15, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 13:00
Opponent
Supervisors
Available from: 2007-01-23 Created: 2007-01-23Bibliographically approved
List of papers
1. Simulation of chromatographic processes applied to separation of proteins
Open this publication in new window or tab >>Simulation of chromatographic processes applied to separation of proteins
1999 In: Journal of Chromatography A, ISSN 00219673, Vol. 846, no 1, 1-12 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-24069 (URN)
Note
Part of urn:nbn:se:su:diva-6582Available from: 2007-01-23 Created: 2007-01-23Bibliographically approved
2. Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography
Open this publication in new window or tab >>Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography
Show others...
2006 In: Biotechnology and Bioengineering, ISSN 00063592, Vol. 93, no 4, 656-664 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-24070 (URN)
Note
Part of urn:nbn:se:su:diva-6582Available from: 2007-01-23 Created: 2007-01-23Bibliographically approved
3. Novel Method for the synthesis of molecularly imprinted polymer bead libraries
Open this publication in new window or tab >>Novel Method for the synthesis of molecularly imprinted polymer bead libraries
2004 In: Macromolecular Rapid Communications, ISSN 10221336, Vol. 25, no 1, 315-320 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-24071 (URN)
Note
Part of urn:nbn:se:su:diva-6582Available from: 2007-01-23 Created: 2007-01-23Bibliographically approved
4. Development and evaluation of spherical molecularly imprinted polymer beads
Open this publication in new window or tab >>Development and evaluation of spherical molecularly imprinted polymer beads
2006 In: Analytical Chemistry, ISSN 00032700, Vol. 78, no 11, 3659-3666 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-24072 (URN)
Note
Part of urn:nbn:se:su:diva-6582Available from: 2007-01-23 Created: 2007-01-23Bibliographically approved

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