Change search
ReferencesLink to record
Permanent link

Direct link
Myotube Formation on UV-lithographically Micro-patterned Glass: A New Experimental System for Studies of Muscle differentiation in vitro
Stockholm University, Faculty of Science, Wenner-Gren Institute for Experimental Biology.
Show others and affiliations
Article in journal (Refereed) Submitted
URN: urn:nbn:se:su:diva-24134OAI: diva2:196839
Part of urn:nbn:se:su:diva-6668Available from: 2007-03-01 Created: 2007-02-22Bibliographically approved
In thesis
1. Adrenergic signaling in insulin-sensitive tissues
Open this publication in new window or tab >>Adrenergic signaling in insulin-sensitive tissues
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Glucose metabolism in insulin-sensitive tissues such as skeletal muscle and adipose tissue is tightly regulated by external stimuli. Metabolic changes in these tissues have direct effects on whole body metabolism. Such metabolic changes can be induced or influenced by adrenergic stimulation.

In L6 skeletal muscle cells, we have seen that the β2-adrenergic receptor increases glycogen synthesis to the same extent as insulin. The β2-adrenergically mediated effect is independent of cyclic AMP but dependent on PI3K.

In brown adipocytes, our data suggest that signaling from the β-adrenergic receptors consists of an acute cyclic AMP effect that is rapidly desensitized and then a prolonged signal involving PI3K.

In skeletal muscle cells in culture, we have shown that DPI (a NADPH oxidase inhibitor) increases glucose uptake through a signaling pathway independent of NADPH oxidase and insulin signaling. DPI instead inhibits complex 1 in the mitochondrial respiratory chain, which lowers ATP levels. This activates AMPK, an activator of glucose uptake.

Furthermore, we have developed a model system for ordered fusion of skeletal muscle cells in culture. In this system, differentiating skeletal muscle cells can be studied separately. This system is optimal for microscopy techniques and easily adaptable for micromanipulations. We have seen that the myogenic factor MyoD can have different expression of the protein in different nuclei within the same myotube. This system could be used with advantage for intracellular signaling and metabolic studies.

Place, publisher, year, edition, pages
Stockholm: Wenner-Grens institut för experimentell biologi, 2007. 180 p.
Metabolism, Adrenergic, Glucose, Signaling, Skeletal muscle
National Category
Research subject
Zoological physiology
urn:nbn:se:su:diva-6668 (URN)91-7155-395-9 (ISBN)
Public defence
2007-03-23, sal E306, Arrheniuslaboratorierna, Svante Arrhenius väg 14-18, Stockholm, 13:00
Available from: 2007-03-01 Created: 2007-02-22Bibliographically approved

Open Access in DiVA

No full text

By organisation
Wenner-Gren Institute for Experimental Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 81 hits
ReferencesLink to record
Permanent link

Direct link