A global topology map of the Saccharomyces cerevisiae membrane proteome
2006 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 103, no 30, 11142-11147 p.Article in journal (Refereed) Published
The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to ≈15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.
Place, publisher, year, edition, pages
2006. Vol. 103, no 30, 11142-11147 p.
IdentifiersURN: urn:nbn:se:su:diva-24329DOI: 10.1073/pnas.0604075103OAI: oai:DiVA.org:su-24329DiVA: diva2:197243
Part of urn:nbn:se:su:diva-68752007-05-242007-05-152010-08-31Bibliographically approved