Validation of peptide epitope microarray experiments and extraction of quality data
2007 (English)In: Journal of Immunological Methods, ISSN 0022-1759, Vol. 328, no 1-2, 1-13 p.Article in journal (Refereed) Published
Within the last decade, the development of antigen microarray slides has enabled the simultaneous measurement of serum reactivity to hundreds of peptides in a single biological sample. Despite this considerable scientific progress, many issues remain regarding the quality, analysis and interpretation of the data these slides produce. There is currently no accepted approach to guide data analysis, and researchers use a wide variety of statistical methods and software tools. We designed and implemented a laboratory experiment to assess the reliability and range of measurement of peptide microarray data, and present graphical and statistical procedures for pre-processing so that quality data can be extracted for addressing biological hypotheses.
Place, publisher, year, edition, pages
2007. Vol. 328, no 1-2, 1-13 p.
Peptide microarray; Statistical analysis; Normalization; Mixed models
IdentifiersURN: urn:nbn:se:su:diva-7229OAI: oai:DiVA.org:su-7229DiVA: diva2:197889