Structural determination of the O-antigenic polysaccharide from the verocytotoxin-producing Escherichia coli O176
2008 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 343, no 4, 805-809 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O176 has been determined. Component analysis together with H-1 and C-13 NMR spectroscopy was employed to elucidate the structure. Inter-residue correlations were determined by H-1,H-1 NOESY and H-1, C-13 heteronuclear multiple-bond correlation experiments. The PS is composed of tetrasaccharide repeating units with the following structure: -> 4)-alpha-D-Manp-(1 -> 2)-alpha-D-Manp-(1 -> 2)-beta-D-Manp-(1 -> 3)-alpha-D-GalpNAc-(-> Cross-peaks of low intensity from alpha-linked mannopyranosyl residues were present in the H-1, H-1 TOCSY NMR spectra and further analysis of these showed that they originate from the terminal part of the polysaccharide. Consequently, the biological repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end. The repeating unit of the E coli O176 O-antigen is similar to those from E coli 017 and 077, thereby explaining the reported cross-reactivities between the strains, and identical to that of Salmonella cerro (O:6, 14, 18).
Place, publisher, year, edition, pages
2008. Vol. 343, no 4, 805-809 p.
Escherichia coli, Salmonella cerro, Lipopolysaccharide, NMR, Biological repeating unit
IdentifiersURN: urn:nbn:se:su:diva-24630DOI: 10.1016/j.carres.2008.01.003ISI: 000254720800026OAI: oai:DiVA.org:su-24630DiVA: diva2:197972