A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids
2007 (English)In: Molecular Therapy, ISSN 1525-0016, E-ISSN 1525-0024, Vol. 15, no 10, 1820-1826 p.Article in journal (Refereed) Published
Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is generally a correlation between high efficacy and cytotoxicity for these peptides. Hence, it is of great importance to find new, non-toxic CPPs with more widespread delivery properties. We present a novel CPP, M918, that efficiently translocates various cells in a non-toxic fashion. In line with most other CPPs, the peptide is internalized mainly via endocytosis, and in particular macropinocytosis, but independent of glycosaminoglycans on the cell surface. In addition, in a splice correction assay using antisense peptide nucleic acid (PNA) conjugated via a disulphide bridge to M918 (M918-PNA), we observed a dose-dependent increase in correct splicing, exceeding the effect of other CPPs. Our data demonstrate that M918 is a novel CPP that can be used to translocate different cargoes inside various cells efficiently.
Place, publisher, year, edition, pages
2007. Vol. 15, no 10, 1820-1826 p.
Pep tides, Nucleic acids, Proteins, Cell membranes, Endocytosis
IdentifiersURN: urn:nbn:se:su:diva-24649DOI: 10.1038/sj.mt.6300255ISI: 000249778000016OAI: oai:DiVA.org:su-24649DiVA: diva2:197995