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The effect of thermal stress on protein composition in dogwhelks (Nucella lapillus) under normoxic and hyperoxic conditions
Stockholm University, Faculty of Science, Department of Systems Ecology.
Stockholm University, Faculty of Science, Department of Systems Ecology.ORCID iD: 0000-0003-2850-3104
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).ORCID iD: 0000-0001-9522-5824
Stockholm University, Faculty of Science, Department of Systems Ecology.
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2007 (English)In: Comparative Biochemistry and Physiology A, ISSN 1095-6433, E-ISSN 1531-4332, Vol. 148, no 4, p. 869-875Article in journal (Refereed) Published
Abstract [en]

In this laboratory study, dogwhelks (Nucella lapillus) were collected from the intertidal zone and exposed to 16 °C (ambient), 26.5 °C and 30 °C under normal and hyperoxic conditions respectively. It was shown that there was no thermally induced mortality at 26.5 °C, but that the mortality rate was 40–50% in 30 °C. This mortality rate was reduced to 10% if extra oxygen was provided, indicating that oxygen supply was setting the limit for whole organism thermal tolerance. Tissue samples were then analysed for protein features using two-dimensional gel electrophoresis, and both up and down regulation of proteins were visualised by silver staining and crosswise comparisons of gels from control vs. treated animals. The results clearly show that the protein profiles from dogwhelks exposed to increased water temperatures differ from those of the control, but that increased oxygen availability alleviates these differences thus increasing the similarity between heat-shocked and control animal protein pattern. This implies a more stable protein metabolism and might explain the increased survival of heat-shocked individuals when extra oxygen is supplied.

Place, publisher, year, edition, pages
2007. Vol. 148, no 4, p. 869-875
Keywords [en]
Nucella, Oxygen, Proteomics, Thermal tolerance
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-24659DOI: 10.1016/j.cbpa.2007.08.034OAI: oai:DiVA.org:su-24659DiVA, id: diva2:198032
Available from: 2008-02-07 Created: 2008-02-07 Last updated: 2018-01-24Bibliographically approved
In thesis
1. Coping with environmental stress: from the individual and population perspective
Open this publication in new window or tab >>Coping with environmental stress: from the individual and population perspective
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Natural stress and disturbances are important factors affecting the structure and function of ecosystems. However the magnitude of stress has escalated due to anthropogenic activities. Environmental monitoring and toxicity assessments try to protect ecosystems from unwanted human alterations. The aim of this Doctoral thesis was to increase the understanding of the complex effects that environmental stress has on individuals and invertebrate populations. The low saline environment in the Baltic Sea is perceived as stressful for most organisms living there. In Paper I, it was found that Baltic blue mussels living in the less saline northern Baltic Proper (~5 psu) had lower basal metabolism and were more susceptible to toxic exposure than the mussels in the south (~7 psu). There was no genetic differentiation between the mussels from the northern and southern areas while there were genetic differences between mussels from sites within the respective areas (Paper III), indicating that there is not a simple relationship between the health of the mussels and genetic diversity in the microsatellite loci studied. In Paper IV it was found that the heat tolerance of the intertidal dogwhelk Nucella lapillus is oxygen dependent. Increased oxygen levels resulted in higher survival rate. Protein expression profiles also became more similar to those of the controls, compared to the whelks exposed to high temperature and normal oxygen levels. In Paper V and VI it was found that exposure to a single toxicant for more than one generation decreased the genetic diversity in exposed copepod populations even though abundances remained unaltered. In Paper VI, exposure to naturally contaminated sediments, which contained of a mixture of toxicants, did not decrease genetic diversity. However the genetic divergence (FST) within the treatments was very high, probably due to small effective population sizes in the replicates. Likewise in Paper III, the very low blue mussel abundance in the north together with the stressful environment suggests a small effective population in the northern Baltic Proper. In conclusion, my studies show that, measuring effects on several levels, including both functional and structural endpoints will both increase the sensitivity of the tests and increase their ecological relevance.

Place, publisher, year, edition, pages
Stockholm: Systemekologiska institutionen, 2008. p. 37
Keywords
Environmental stress, Genetic diversity, Multi-level approach, Invertebrates
National Category
Ecology
Research subject
Systems Ecology
Identifiers
urn:nbn:se:su:diva-7311 (URN)978-91-7155-566-3 (ISBN)
Public defence
2008-02-29, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 8 A, Stockholm, 10:00
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Available from: 2008-02-07 Created: 2008-02-07Bibliographically approved

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Elfwing, TinaLöf, MarieTedengren, Michael
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