The nucleotide pool is a significant target for oxidative stress
2006 (English)In: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 41, no 4, 620-626 p.Article in journal (Refereed) Published
Oxidative stress is considered to be one of the most important phenomena involved in the process of aging and age-related diseases. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) has been frequently used as a marker for oxidative stress. However, the origin of extracellular 8-oxo-dG is not well understood. The aim of this work was to investigate the nucleotide pool and the role of the human mutT homologue protein (hMTH1) in the appearance of extracellular 8-oxo-dG in a cellular model system. For this purpose we used primary human fibroblast cells, which were transfected by siRNAs homologous to hMTH1. Extracellular 8-oxo-dG in cell culture media after exposure of the cells to ionizing radiation was measured as enzyme-linked immunosorbent assay reactivity. Our results demonstrate the profound effect of both hMTH1 expression and nucleotide pool size on the cellular excretion of 8-oxo-dG, suggesting that the nucleotide pool is a significant target for the formation of extracellular 8-oxo-dG.
Place, publisher, year, edition, pages
Elsevier Inc. , 2006. Vol. 41, no 4, 620-626 p.
8-Oxo-dG; 8-OH-dG; Nucleotide pool; hMTH1; siRNA; Radiation; Oxidative stress; Free radicals
Microbiology in the medical area
IdentifiersURN: urn:nbn:se:su:diva-24837DOI: 10.1016/j.freeradbiomed.2006.05.003OAI: oai:DiVA.org:su-24837DiVA: diva2:198400
Part of urn:nbn:se:su:diva-7492005-11-222005-11-222010-09-15Bibliographically approved