Biogenesis of MalF and the MalFGK2 maltose transport complex in Escherichia coli requires YidC
2008 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, no 283, 17881-17890 p.Article in journal (Refereed) Published
The polytopic inner membrane protein MalF is a constituent of the MalFGK2 maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK2 maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.
Place, publisher, year, edition, pages
2008. no 283, 17881-17890 p.
Membrane Transport, Structure, Function, and Biogenesis
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-24886DOI: 10.1074/jbc.M801481200OAI: oai:DiVA.org:su-24886DiVA: diva2:198471