NMR investigations of interactions between anesthetics and lipid bilayers
2008 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, Vol. 1178, no 11, 2604-2611 p.Article in journal (Refereed) Published
Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional 1H–13C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocaine molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P–N vector in the choline group.
Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2008. Vol. 1178, no 11, 2604-2611 p.
Solid-state NMR, Carbon-13, Phosphorus-31, Deuterium, Dipolar couplings, Separated local field, Quadrupolar coupling, Order parameter, Lyotropic liquid crystal, Biomembrane, Anesthetic
Research subject Physical Chemistry
IdentifiersURN: urn:nbn:se:su:diva-25065DOI: 10.1016/j.bbamem.2008.07.023ISI: 000261263500018OAI: oai:DiVA.org:su-25065DiVA: diva2:198824
Part of urn:nbn:se:su:diva-76812008-05-132008-05-132010-01-28Bibliographically approved