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NMR investigations of interactions between anesthetics and lipid bilayers
Stockholm University, Faculty of Science, Department of Physical, Inorganic and Structural Chemistry.
Stockholm University, Faculty of Science, Department of Physical, Inorganic and Structural Chemistry.
Division of Physical Chemistry, Royal Institute of Technology, SE-10044 Stockholm, Sweden.
Stockholm University, Faculty of Science, Department of Physical, Inorganic and Structural Chemistry.
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2008 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1178, no 11, 2604-2611 p.Article in journal (Refereed) Published
Abstract [en]

Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional 1H–13C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocaine molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P–N vector in the choline group.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2008. Vol. 1178, no 11, 2604-2611 p.
Keyword [en]
Solid-state NMR, Carbon-13, Phosphorus-31, Deuterium, Dipolar couplings, Separated local field, Quadrupolar coupling, Order parameter, Lyotropic liquid crystal, Biomembrane, Anesthetic
National Category
Physical Chemistry
Research subject
Physical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-25065DOI: 10.1016/j.bbamem.2008.07.023ISI: 000261263500018OAI: oai:DiVA.org:su-25065DiVA: diva2:198824
Note
Part of urn:nbn:se:su:diva-7681Available from: 2008-05-13 Created: 2008-05-13 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Solid-State NMR Characterization of Lipid Membranes
Open this publication in new window or tab >>Solid-State NMR Characterization of Lipid Membranes
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis deals with the study of lipid bilayer systems by solid-state nuclear magnetic resonance. Two-dimensional 1H-13C separated local field experiments under magic-angle spinning were employed to investigate structural and dynamical modifications of cell membranes, resulting from the addition of compounds with some biological relevance. For further interpretation of the segmental order obtained from the 2D experiments other methods, such as 31P-NMR, 2H-NMR and molecular dynamics simulations, were also employed.

The work presented in this thesis can be divided into two parts. The first part refers to the setup of experimental conditions. Heating and hydration effects were addressed in order to define both the temperature of the system as well as the number of water molecules per lipid necessary to fully hydrate the bilayer. Application of this experimental setup to lipid membrane systems with biological relevance constitutes the second part. The interaction of monogalactosyl- diacylglycerol, the most abundant lipid on earth, with dimyristoylphosphatidylcholine, was studied, which resulted in a frustrated bilayer. Furthermore, small molecules of local anesthetics, with focus on lidocaine, were inserted in the model membrane with the purpose of understanding the influence of local anesthetics on lipid bilayers.

Place, publisher, year, edition, pages
Stockholm: Institutionen för fysikalisk kemi, oorganisk kemi och strukturkemi, 2008. 69 p.
Keyword
Solid-State NMR, Membranes, Lipids, Glycolipids, Local Anesthetics, Hydration
National Category
Physical Chemistry
Research subject
Physical Chemistry
Identifiers
urn:nbn:se:su:diva-7681 (URN)978-91-7155-659-2 (ISBN)
Public defence
2008-06-03, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 13:00
Opponent
Supervisors
Available from: 2008-05-13 Created: 2008-05-13Bibliographically approved

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