Functional features of the C-terminal region of yeast ribosomal protein L5
2008 (English)In: Molecular Genetics and Genomics, ISSN 1617-4615, E-ISSN 1617-4623, Vol. 280, no 4, 337-350 p.Article in journal (Refereed) Published
The aim of this study was to analyze the functional importance of the C-terminus of the essential yeast ribosomal protein L5 (YrpL5). Previous studies have indicated that the C-terminal region of YrpL5 forms an α-helix with a positively charged surface that is involved in protein–5S rRNA interaction. Formation of an YrpL5·5S rRNA complex is a prerequisite for nuclear import of YrpL5. Here we have tested the importance of the α-helix and the positively charged surface for YrpL5 function in Saccharomyces cerevisiae using site directed mutagenesis in combination with functional complementation. Alterations in the sequence forming the putative α-helix affected the functional capacity of YrpL5. However, the effect did not correlate with a decreased ability of the protein to bind to 5S rRNA as all rpL5 mutants tested were imported to the nucleus whether or not the α-helix or the positively charged surface were intact. The alterations introduced in the C-terminal sequence affected the growth rate of cells expressing mutant but functional forms of YrpL5. The reduced growth rate was correlated with a reduced ribosomal content per cell indicating that the alterations introduced in the C-terminus interfered with ribosome assembly.
Place, publisher, year, edition, pages
2008. Vol. 280, no 4, 337-350 p.
Functional complementation, Mutation analysis, Ribosomal protein L5, S. cerevisiae
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-25114DOI: 10.1007/s00438-008-0369-7ISI: 000258902300007OAI: oai:DiVA.org:su-25114DiVA: diva2:198931