Alzheimer amyloid-beta peptides block the activation of C/EBP beta and C/EBP delta in glial cells
Stockholm University, Faculty of Science, Department of Neurochemistry2008 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 370, no 4, 619-622 p.Article in journal (Refereed) Published
Members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors have been reported to be up-regulated in Alzheimer's disease. In the present study, we have investigated the effects of amyloid-beta (A beta) peptides on C/EBP beta and C/EBP delta, previously shown to be induced by inflammatory stimuli in glial cells. Surprisingly, electrophoretic mobility shift assay showed that both A beta(1-42) and A beta(25-35) blocked C/EBP activation induced by the inflammatory cytokine interleukin-1 (IL-1 beta) or lipopolysac-charicle (LPS) in mixed primary glial cell cultures from rat. A beta also blocked IL-1 or LPS-incluced C/EBP protein levels. The most prominent effects were observed on DNA binding activity and protein levels of C/EBP delta. Our results demonstrate a dysregulation of C/EBP when glial cells are activated in the presence of Alzheimer A beta peptides.
Place, publisher, year, edition, pages
2008. Vol. 370, no 4, 619-622 p.
Alzheimer's disease, amyloid-beta, C/EBP, Glia, neuroinflammation
Biochemistry and Molecular Biology Biophysics
IdentifiersURN: urn:nbn:se:su:diva-25295DOI: 10.1016/j.bbrc.2008.03.150ISI: 000255883900018OAI: oai:DiVA.org:su-25295DiVA: diva2:199364