Characterization of the site-specific recombination system of phage ΦD145, and its capacity to promote recombination in human cells
2010 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 408, no 1, 64-70 p.Article in journal (Refereed) Published
Phage integrases have the potential of becoming tools for safe site-specific integration of genes into unmodified human genomes. The P2-like phages have been found to have different bacterial host integration sites and consequently they have related integrases with different sequence specificities. In this work the site-specific recombination system of the P2-like phage ΦD145 is characterized. The minimal attB site is determined to 22 nt with 18 nt identity to the core region of attP. A non-coding sequence on the human chromosome 13 is shown to be a rather good substrate for recombination in vivo in bacteria as well as in a plasmid system in HeLa cells when HMG protein recognition sequences are inserted between the left arm-binding site and the core in the complex phage attachment site attP. Thus ΦD145 integrase that belongs to the tyrosine family shows potential as a tool for site-specific integration into the human genome.
Place, publisher, year, edition, pages
2010. Vol. 408, no 1, 64-70 p.
Integrase, Site-specific recombination, Bacteriophage
IdentifiersURN: urn:nbn:se:su:diva-25724DOI: 10.1016/j.virol.2010.08.035ISI: 000283970500007OAI: oai:DiVA.org:su-25724DiVA: diva2:200376