Membrane topology of the human seipin protein
2006 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 580, no 9, 2281-2284 p.Article in journal (Refereed) Published
The Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) gene encodes an integral membrane protein, called seipin, of unknown function localized to the endoplasmic reticulum of eukaryotic cells. Seipin is associated with the heterogeneous genetic disease BSCL2, and mutations in an N-glycosylation motif links the protein to two other disorders, autosomal-dominant distal hereditary motor neuropathy type V and Silver syndrome. Here, we report a topological study of seipin using an in vitro topology mapping assay. Our results suggest that the predominant form of seipin is 462 residues long and has an N-cyt-C-cyt orientation with a long luminal loop between the two transmembrane helices.
Place, publisher, year, edition, pages
2006. Vol. 580, no 9, 2281-2284 p.
seipin, N-linked glycosylation, oligosaccharyl transferase, topology mapping, BCSL gene
IdentifiersURN: urn:nbn:se:su:diva-25793DOI: 10.1016/j.febslet.2006.03.040ISI: 000237012800021OAI: oai:DiVA.org:su-25793DiVA: diva2:200525