Membrane topology of the Drosophila OR83b odorant receptor
2007 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 581, no 29, 5601-5604 p.Article in journal (Refereed) Published
By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster, have long been thought to belong to the G-protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical N-out - C-in 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N-terminus, an extracellular C-terminus, and 7TM helices. (c) 2007 Federation of European Biochemical Societies.
Place, publisher, year, edition, pages
2007. Vol. 581, no 29, 5601-5604 p.
odorant receptor, membrane protein, topology, Drosophila melanogaster
IdentifiersURN: urn:nbn:se:su:diva-25794DOI: 10.1016/j.febslet.2007.11.007ISI: 000253488000010OAI: oai:DiVA.org:su-25794DiVA: diva2:200526