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The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2005 In: Biochimica et Biophysica Acta, Vol. 1707, no 2-3, 179-88 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2005. Vol. 1707, no 2-3, 179-88 p.
URN: urn:nbn:se:su:diva-25799OAI: diva2:200545
Part of urn:nbn:se:su:diva-861Available from: 2006-03-03 Created: 2006-03-03Bibliographically approved
In thesis
1. Novel Technologies for Recombinant Protein Overexpression in Escherichia coli
Open this publication in new window or tab >>Novel Technologies for Recombinant Protein Overexpression in Escherichia coli
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The use of recombinant protein is a cornerstone in many structural and functional studies. The enteric bacterium Escherichia Coli is the most commonly used organism for producing recombinant proteins. E. coli has several advantages over other expression hosts, but also one major disadvantage - the protein of interest does not always adopt its native conformation. Instead the protein might form large insoluble aggregates, inclusion bodies, within the cell. In particular, the heterologous overexpression of eukaryotic and membrane proteins are troublesome.

In this thesis, methods are described that can be used to increase the likelihood of overexpressing eukaryotic proteins as well as membrane proteins. In particular, a novel method is described that can distinguish between bacterial colonies expressing soluble proteins from those expressing inclusion bodies. The method utilizes the fact that inclusion bodies are of a considerable size and can be removed by filtration. Using this screening method in combination with methods that alter the physical properties of proteins, we have shown that the likelihood of overexpression in E. coli can be dramatically increased.

Place, publisher, year, edition, pages
Stockholm: Institutionen för biokemi och biofysik, 2006. 76 p.
National Category
Biochemistry and Molecular Biology
urn:nbn:se:su:diva-861 (URN)91-7155-212-X (ISBN)
Public defence
2006-03-24, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 14:00 (English)
Available from: 2006-03-03 Created: 2006-03-03 Last updated: 2010-01-13Bibliographically approved

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