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Multiple neurotransmitter inputs modulate circadian clock neurons in Drosophila
Stockholm University, Faculty of Science, Department of Zoology.
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Most animals have endogenous circadian clocks, which drive daily rhythms in behavior and physiology to adapt to daily cycles of the environment. Several sets of neurons have been identified as circadian pacemakers (clocks) in the brain of Drosophila melanogaster, but the neuronal circuits and neurotransmitters signalling circadian information are poorly known. The current study aimed at identifying neurons and neurotransmitters that form inputs to circadian clock neurons of Drosophila.

Immunochytochemistry and the GAL4-UAS system were used to detect neurotransmitters in neurons possibly contacting the clock neurons. Axonal processes containing acetylcholine, GABA, serotonin (5-HT) and glutamate are in close proximity to dendrites of the major clock neurons, the s-LNvs. To test the action of these transmitters on the s-LNvs, we employed calcium imaging on dissociated GFP-labeled larval s-LNvs. We found that acetylcholine induced excitatory, while 5-HT, glutamate and GABA produced inhibitory responses in intracellular calcium levels of the LNvs. Pharmacological experiments revealed that the receptors for acetylcholine are of ion channel type (nicotinic), while GABA and glutamate act on metabotropic G-protein coupled receptors (designated GABAB-R and DmGluRA). These receptors were identified on the s-LNvs by immunochytochemistry in the intact brain and on dissociated neurons.

We investigated functions of the GABAB-R and DmGluRA in the activity rhythms using transgenic flies with altered receptor expressions. Although GABAB-R RNA interference failed to produce altered behavioral phenotypes, the DmGluRA interference indicated that the glutamate signaling to s-LNvs modulates the activity pattern under light-dark conditions. In conclusion, the major circadian clock neurons, the s-LNvs, receive multiple inputs via specific receptors for acetylcholine, GABA, glutamate and 5-HT. Possible roles of these inputs are discussed in relation to circadian rhythms of Drosophila.

Place, publisher, year, edition, pages
Stockholm: Zoologiska institutionen , 2006.
Keyword [en]
circadian, Drosophila, neurotransmitter, G-protein+coupled receptor
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:su:diva-947ISBN: 91-7155-224-3 (print)OAI: oai:DiVA.org:su-947DiVA: diva2:200696
Public defence
2006-04-24, sal G, Arrheniuslaboratorierna, Svante Arrhenius väg 14-18, Stockholm, 10:00 (English)
Opponent
Supervisors
Available from: 2006-04-03 Created: 2006-04-03 Last updated: 2010-01-13Bibliographically approved
List of papers
1. Acetylcholine increases intracellular Ca2+ via nicotinic receptors in cultured PDF-containing clock neurons of Drosophila
Open this publication in new window or tab >>Acetylcholine increases intracellular Ca2+ via nicotinic receptors in cultured PDF-containing clock neurons of Drosophila
2004 (English)In: Journal of neurophysiology, ISSN 0022-3077, Vol. 91, no 2, 912-923 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25855 (URN)
Note
Part of urn:nbn:se:su:diva-947Available from: 2006-04-03 Created: 2006-04-03 Last updated: 2014-10-28Bibliographically approved
2. GABA modulates Drosophila circadian clock neurons via GABAB receptors and decreases in calcium
Open this publication in new window or tab >>GABA modulates Drosophila circadian clock neurons via GABAB receptors and decreases in calcium
2005 (English)In: Journal of Neurobiology, ISSN 0022-3034, Vol. 65, no 3, 225-240 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25856 (URN)
Note
Part of urn:nbn:se:su:diva-947Available from: 2006-04-03 Created: 2006-04-03 Last updated: 2014-10-28Bibliographically approved
3. Mapping of serotonin, dopamine, and histamine in relation to different clock neurons in the brain of Drosophila
Open this publication in new window or tab >>Mapping of serotonin, dopamine, and histamine in relation to different clock neurons in the brain of Drosophila
2006 (English)In: The Journal of Comparative Neurology, ISSN 0021-9967, Vol. 494, no 2, 314-330 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25857 (URN)
Note
Part of urn:nbn:se:su:diva-947Available from: 2006-04-03 Created: 2006-04-03 Last updated: 2014-10-28Bibliographically approved
4. Glutamate and its metabotropic receptor in Drosophila clock neuron circuits
Open this publication in new window or tab >>Glutamate and its metabotropic receptor in Drosophila clock neuron circuits
Show others...
2007 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 505, no 1, 32-45 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-19457 (URN)10.1002/cne.21471 (DOI)000250117800003 ()17729267 (PubMedID)
Available from: 2007-11-12 Created: 2007-11-12 Last updated: 2017-12-13Bibliographically approved

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